ARG45149
anti-Dicer antibody
anti-Dicer antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Dicer |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Dicer |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human Dicer. |
| Conjugation | Un-conjugated |
| Alternate Names | DICER1; dicer 1, ribonuclease type III; MNG1; Dicer1e; HERNA; DCR1; EC 3.1.26.3; Helicase with RNase motif; Endoribonuclease Dicer; Helicase MOI; Dicer; K12H4.8-LIKE; RMSE2; KIAA0928; Multinodular Goitre 1; EC 3.1.26 47; RMSE2; GLOW; AviD |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Observed Size | 250 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | DICER1 |
| Gene Full Name | dicer 1, ribonuclease type III |
| Background | This gene encodes a protein possessing an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus. The encoded protein functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. This protein also acts as a strong antiviral agent with activity against RNA viruses, including the Zika and SARS-CoV-2 viruses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2021] |
| Function | Double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. Cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a mean to specifically regulate the expression of target genes. [UniProt] |
| Cellular Localization | Cytoplasm; Cytoplasm, perinuclear region. [UniProt] |
| Calculated MW | 219 kDa |
| PTM | Phosphoprotein. [UniProt] |
Images (5) Click the Picture to Zoom In
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ARG45149 anti-Dicer antibody IHC-P image
Immunohistochemistry: Human breast cancer tissue stained with ARG45149 anti-Dicer antibody at 2 μg/ml dilution.
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ARG45149 anti-Dicer antibody WB image
Western blot: 293T stained with ARG45149 anti-Dicer antibody at 0.5 μg/ml dilution.
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ARG45149 anti-Dicer antibody FACS image
Flow Cytometry: MCF-7 stained with ARG45149 anti-Dicer antibody at 1 µg/10^6 cells dilution.
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ARG45149 anti-Dicer antibody WB image
Western blot: C6 stained with ARG45149 anti-Dicer antibody at 0.5 μg/ml dilution.
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ARG45149 anti-Dicer antibody WB image
Western blot: Mouse brain and NIH/3T3 stained with ARG45149 anti-Dicer antibody at 0.5 μg/ml dilution.
