ARG20541

anti-Dnmt1 antibody [60B1220.1]

anti-Dnmt1 antibody [60B1220.1] for ChIP,Immunohistochemistry,Immunoprecipitation,Western blot and Fish,Human,Mouse,Zebrafish

Gene Regulation antibody

Overview

Product Description

Mouse Monoclonal antibody [60B1220.1] recognizes Dnmt1

Tested Reactivity Hu, Ms, Fsh, Zfsh
Tested Application ChIP, IHC, IP, WB
Specificity Cross-react with mouse DNMT1.
Host Mouse
Clonality Monoclonal
Clone 60B1220.1
Isotype IgG1 Kappa
Target Name Dnmt1
Antigen Species Human
Immunogen Synthetic peptide around aa. 637-650 of Human DNMT1.
Conjugation Un-conjugated
Full Name DNA (cytosine-5-)-methyltransferase 1
Alternate Names AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN; DNA (cytosine-5)-methyltransferase 1; Dnmt1; EC 2.1.1.37; CXXC-type zinc finger protein 9; DNA methyltransferase HsaI; DNA MTase HsaI; M.HsaI; MCMT

Application Instructions

Application Suggestion
Tested Application Dilution
ChIPAssay-dependent
IHCAssay-dependent
IPAssay-dependent
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 183 kDa

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS, 0.05% BSA and 0.05% Sodium azide
Preservative 0.05% Sodium azide
Stabilizer 0.05% BSA
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 13433 Mouse DNMT1

GeneID: 1786 Human DNMT1

Swiss-port # P13864 Mouse DNA (cytosine-5)-methyltransferase 1

Swiss-port # P26358 Human DNA (cytosine-5)-methyltransferase 1

Gene Symbol DNMT1
Gene Full Name DNA (cytosine-5-)-methyltransferase 1
Background Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2F-binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells.
Function Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. [UniProt]
Cellular Localization Nucleus
Research Area Gene Regulation antibody
PTM Sumoylated; sumoylation increases activity.
Acetylation on multiple lysines, mainly by KAT2B/PCAF, regulates cell cycle G(2)/M transition. Deacetylation of Lys-1349 and Lys-1415 by SIRT1 increases methyltransferase activity.
Phosphorylation of Ser-154 by CDKs is important for enzymatic activity and protein stability. Phosphorylation of Ser-143 by AKT1 prevents methylation by SETD7 therebye increasing DNMT1 stability.
Methylation at Lys-142 by SETD7 promotes DNMT1 proteasomal degradation.
Ubiquitinated by UHRF1; interaction with USP7 counteracts ubiquitination by UHRF1 by promoting deubiquitination and preventing degradation by the proteasome.

Images (3) Click the Picture to Zoom In

  • ARG20541 anti-Dnmt1 antibody [60B1220.1] IHC image

    Immunohistochemistry: Medullar kidney tissue sections stained with ARG20541 anti-Dnmt1 antibody [60B1220.1].

  • ARG20541 anti-Dnmt1 antibody [60B1220.1] WB image

    Western blot: Human H1299 cell lysate stained with ARG20541 anti-Dnmt1 antibody [60B1220.1] at 1:1000 dilution.

  • ARG20541 anti-Dnmt1 antibody [60B1220.1] IHC image

    Immunohistochemistry: Formalin-fixed Human colon carcinoma stained with ARG20541 anti-Dnmt1 antibody [60B1220.1] at 1:10,000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Magnification: 40x.