ARG54845

anti-Dnmt3b antibody

anti-Dnmt3b antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

Gene Regulation antibody
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Overview

Product Description

Rabbit Polyclonal antibody recognizes Dnmt3b

Tested Reactivity Hu, Ms
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Dnmt3b
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide corresponding to aa. 389-417 of Human Dnmt3b.
Conjugation Un-conjugated
Alternate Names ICF; ICF1; M.HsaIIIB; DNA (cytosine-5)-methyltransferase 3B; Dnmt3b; EC 2.1.1.37; DNA methyltransferase HsaIIIB; DNA MTase HsaIIIB; M.HsaIIIB

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-PAssay-dependent
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS and 0.09% (W/V) Sodium azide
Preservative 0.09% (W/V) Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1789 Human DNMT3B

Swiss-port # Q9UBC3 Human DNA (cytosine-5)-methyltransferase 3B

Gene Symbol DNMT3B
Gene Full Name DNA (cytosine-5-)-methyltransferase 3 beta
Background CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Eight alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined. [provided by RefSeq, May 2011]
Function Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation. Seems to be involved in gene silencing (By similarity). In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Isoforms 4 and 5 are probably not functional due to the deletion of two conserved methyltransferase motifs. Function as transcriptional corepressor by associating with ZHX1. [UniProt]
Cellular Localization Nucleus
Research Area Gene Regulation antibody
Calculated MW 96 kDa
PTM Sumoylated.
Citrullinated by PADI4.

Images (2) Click the Picture to Zoom In

  • ARG54845 anti-Dnmt3b antibody WB image

    Western blot: 30 µg of HCT116 and Mouse testis lysates stained with ARG54845 anti-Dnmt3b antibody at 1:1000 dilution.

  • ARG54845 anti-Dnmt3b antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human prostata carcinoma tissue stained with ARG54845 anti-Dnmt3b antibody.

Customer's Feedback

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Review for anti-Dnmt3b antibody

Application:WB

Sample:HCT116 and Mouse testis

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC