ARG52264

anti-Dopamine Transporter antibody, Extracellular Loop 2

anti-Dopamine Transporter antibody, Extracellular Loop 2 for Western blot,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections and Human,Primates

Neuroscience antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes Dopamine Transporter
Tested Reactivity Hu, NHuPrm
Tested Application IHC-Fr, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Dopamine Transporter
Antigen Species Human
Immunogen Synthetic peptide corresponding to amino acid residues from the extracellular loop 2 region conjugated to KLH
Conjugation Un-conjugated
Alternate Names PKDYS; Sodium-dependent dopamine transporter; Solute carrier family 6 member 3; DAT1; DAT; DA transporter

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-FrAssay-dependent
IHC-PAssay-dependent
WB1:1,000
Application Note Specific for the ~88k DAT protein in Western blots.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity Purified
Buffer 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol
Stabilizer 0.1 mg/ml BSA, 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 6531 Human SLC6A3

Swiss-port # Q01959 Human Sodium-dependent dopamine transporter

Gene Symbol SLC6A3
Gene Full Name solute carrier family 6 (neurotransmitter transporter), member 3
Background The dopamine transporter (DAT) is responsible for the reaccumulation of dopamine after it has been released. DAT antibodies and antibodies for other markers of catecholamine biosynthesis are widely used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). Levels of DAT protein expression are altered by chronic drug administration (Wilson et al., 1996).
Research Area Neuroscience antibody
Calculated MW 68 kDa

Images (1) Click the Picture to Zoom In

  • ARG52264 anti-Dopamine Transporter antibody, Extracellular Loop 2 WB image

    Western blot: human caudate lysate stained with ARG52264 anti-Dopamine Transporter antibody, Extracellular Loop 2 showing specific immunolabeling of the ~88k DAT protein.