ARG52267
anti-DsbA-L antibody
anti-DsbA-L antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cell Biology and Cellular Response antibody; Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes DsbA-L |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | DsbA-L |
| Antigen Species | Mouse |
| Immunogen | Full length recombinant mouse DsbA-L protein |
| Conjugation | Un-conjugated |
| Alternate Names | Glutathione S-transferase kappa 1; hGSTK1; GST; GST class-kappa; GST13-13; EC 2.5.1.18; Glutathione S-transferase subunit 13; GST13; GST 13-13; GSTK1-1 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | Specific for the ~25 kDa DsbA-L protein in Western blots of 3T3-L1 adipocytes and mouse white and brown adipose tissue lysate samples. It is suggested that you add 1% BSA to the antibody dilution buffer. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Serum |
| Buffer | Serum and 40 % Glycerol |
| Stabilizer | 40 % Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Gstk1 |
| Gene Full Name | glutathione S-transferase kappa 1 |
| Background | Disulfide-bond-A oxidoreductase-like protein (DsbA-L, previously named as GST Kappa) is an adiponectin-interacting protein. DsbA-L is highly expressed in adipose tissue, and its expression level is negatively correlated with obesity in mice and humans. DsbA-L expression in 3T3-L1 adipocytes is stimulated by the insulin sensitizer rosiglitazone and inhibited by the inflammatory cytokine TNFalpha. Polymorphism of DsbA-L gene has recently been implicated in insulin secretion and body fat distribution (Gao F et al., 2009). Overexpression of DsbA-L promotes adiponectin multimerization while suppressing DsbA-L expression by RNAi markedly and selectively reduces adiponectin levels and secretion in 3T3-L1 adipocytes. Recent studies identify DsbA-L as a key regulator for adiponectin biosynthesis (Liu et al., 2008). |
| Research Area | Cell Biology and Cellular Response antibody; Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 25 kDa |
Images (1) Click the Picture to Zoom In
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ARG52267 anti-DsbA-L antibody WB image
Western Blot: mouse adipose tissue showing specific immunolabeling of the ~25k DsbA-L protein stained with DsbA-L antibody (ARG52267).
