ARG52267

anti-DsbA-L antibody

anti-DsbA-L antibody for Western blot,Immunohistochemistry and Human,Mouse

Cell Biology and Cellular Response antibody; Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes DsbA-L
Tested Reactivity Hu, Ms
Tested Application IHC, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name DsbA-L
Antigen Species Mouse
Immunogen Full length recombinant mouse DsbA-L protein
Conjugation Un-conjugated
Alternate Names Glutathione S-transferase kappa 1; hGSTK1; GST; GST class-kappa; GST13-13; EC 2.5.1.18; Glutathione S-transferase subunit 13; GST13; GST 13-13; GSTK1-1

Application Instructions

Application Suggestion
Tested Application Dilution
IHC1:200
WB1:2000
Application Note Specific for the ~25 kDa DsbA-L protein in Western blots of 3T3-L1 adipocytes and mouse white and brown adipose tissue lysate samples. It is suggested that you add 1% BSA to the antibody dilution buffer.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Serum
Buffer Serum and 40 % Glycerol
Stabilizer 40 % Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 373156 Human GSTK1

GeneID: 76263 Mouse GSTK1

Swiss-port # Q9DCM2 Mouse Glutathione S-transferase kappa 1

Swiss-port # Q9Y2Q3 Human Glutathione S-transferase kappa 1

Gene Symbol Gstk1
Gene Full Name glutathione S-transferase kappa 1
Background Disulfide-bond-A oxidoreductase-like protein (DsbA-L, previously named as GST Kappa) is an adiponectin-interacting protein. DsbA-L is highly expressed in adipose tissue, and its expression level is negatively correlated with obesity in mice and humans. DsbA-L expression in 3T3-L1 adipocytes is stimulated by the insulin sensitizer rosiglitazone and inhibited by the inflammatory cytokine TNFalpha. Polymorphism of DsbA-L gene has recently been implicated in insulin secretion and body fat distribution (Gao F et al., 2009). Overexpression of DsbA-L promotes adiponectin multimerization while suppressing DsbA-L expression by RNAi markedly and selectively reduces adiponectin levels and secretion in 3T3-L1 adipocytes. Recent studies identify DsbA-L as a key regulator for adiponectin biosynthesis (Liu et al., 2008).
Research Area Cell Biology and Cellular Response antibody; Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody
Calculated MW 25 kDa

Images (1) Click the Picture to Zoom In

  • ARG52267 anti-DsbA-L antibody WB image

    Western Blot: mouse adipose tissue showing specific immunolabeling of the ~25k DsbA-L protein stained with DsbA-L antibody (ARG52267).