ARG57526

anti-E Cadherin antibody [4A2]

anti-E Cadherin antibody [4A2] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

EMT Study antibody; Epithelial Marker antibody

Overview

Product Description Mouse Monoclonal antibody [4A2] recognizes E Cadherin
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, ICC/IF, IHC-P, WB
Host Mouse
Clonality Monoclonal
Clone 4A2
Isotype IgG1, kappa
Target Name E Cadherin
Antigen Species Human
Immunogen Recombinant protein of Human E-Cadherin.
Conjugation Un-conjugated
Full Name cadherin 1, type 1
Alternate Names Uvomorulin; Arc-1; Cadherin-1; E-cadherin; CDHE; CD antigen CD324; ECAD; CAM 120/80; LCAM; Epithelial cadherin; UVO; CD324

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 2 µg/10^6 cells
ICC/IF1 - 4 µg/ml
IHC-P1 - 2 µg/ml
WB1 - 2 µg/ml
Application Note IHC-P: Antigen Retrieval: Boil tissue section in 10mM Citrate buffer (pH 6.0) for 10-20 min followed by cooling at RT for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 97 kDa
Observed Size 135 kDa (precursor), 80-120 kDa (mature, depending on gylcosylation level).

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS, 0.05% Sodium azide and 0.1 mg/ml BSA.
Preservative 0.05% Sodium azide
Stabilizer 0.1 mg/ml BSA
Concentration 0.2 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 12550 Mouse CDH1

GeneID: 83502 Rat CDH1

GeneID: 999 Human CDH1

Gene Symbol CDH1
Gene Full Name cadherin 1, type 1
Background E Cadherin is a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16. [provided by RefSeq, Nov 2015]
Function Cadherins are calcium-dependent cell adhesion proteins (PubMed:11976333). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed:11976333). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.

E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.

(Microbial infection) Serves as a receptor for Listeria monocytogenes; internalin A (InlA) binds to this protein and promotes uptake of the bacteria. [UniProt]
Cellular Localization Cell junction
Cell membrane; Single-pass type I membrane protein
Endosome
Golgi apparatus; trans-Golgi network [UniProt]
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Anti-Mouse IgG secondary antibodies;
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Tools for studying H. pylori diseases
Research Area EMT Study antibody; Epithelial Marker antibody
PTM During apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3 to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm. The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway. Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system. The gamma-secretase-mediated cleavage promotes disassembly of adherens junctions.

N-glycosylation at Asn-637 is essential for expression, folding and trafficking.

Ubiquitinated by a SCF complex containing SKP2, which requires prior phosphorylation by CK1/CSNK1A1. Ubiquitinated by CBLL1/HAKAI, requires prior phosphorylation at Tyr-754. [UniProt]

Images (4) Click the Picture to Zoom In

  • ARG57526 anti-E Cadherin antibody [4A2] ICC/IF image

    Immunofluorescence: MCF7 cells stained with ARG57526 anti-E Cadherin antibody [4A2] (green). Reddot (red) for nuclear staining.

  • ARG57526 anti-E Cadherin antibody [4A2] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human skin tissue stained with ARG57526 anti-E Cadherin antibody [4A2]. Antigen Retrieval: Boil tissue section in 10mM Citrate buffer (pH 6.0) for 10-20 min followed by cooling at RT for 20 min.

  • ARG57526 anti-E Cadherin antibody [4A2] WB image

    Western blot: MCF7 cell lysate stained with ARG57526 anti-E Cadherin antibody [4A2].

  • ARG57526 anti-E Cadherin antibody [4A2] FACS image

    Flow Cytometry: MCF7 cells stained with ARG57526 anti-E Cadherin antibody [4A2] (blue) or isotype control (red).