ARG65402

anti-E Cadherin antibody [67A4]

anti-E Cadherin antibody [67A4] for Flow cytometry,ICC/IF,IHC-Frozen sections,Immunoprecipitation,Western blot and Human

Cancer antibody; Developmental Biology antibody; Signaling Transduction antibody
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anti-E Cadherin antibody [67A4] Mouse Monoclonal antibody [67A4] recognizes E Cadherin ARG65402 Arigo biolaboratories
4.0 out of 5 based on 3
anti-E Cadherin antibody [67A4] is rated 4.0 out of 5 by 3.

anti-E Cadherin antibody [67A4]

Overview

Product Description Mouse Monoclonal antibody [67A4] recognizes E Cadherin
Tested Reactivity Hu
Tested Application FACS, ICC/IF, IHC-Fr, IP, WB
Specificity The mouse monoclonal antibody 67A4 recognizes CD324 / Ecadherin, an approximately 100 kDa epithelial cell adhesion molecule, whose detection is important for determination of invasive potential of epithelial neoplasms.
HLDA VIII
Host Mouse
Clonality Monoclonal
Clone 67A4
Isotype IgG1
Target Name E Cadherin
Immunogen T-47D cells_x000D_
Conjugation Un-conjugated
Full Name cadherin 1, type 1, E-cadherin (epithelial)
Alternate Names Uvomorulin; Arc-1; Cadherin-1; E-cadherin; CDHE; CD antigen CD324; ECAD; CAM 120/80; LCAM; Epithelial cadherin; UVO; CD324

Application Instructions

Application Suggestion
Tested Application Dilution
FACSAssay-dependent
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IPAssay-dependent
WB1 - 4 µg/ml
Application Note
Flow Cytometry: Application note:Tested on cell lines CACO-2 and HT-29. In this case the recommended concentration is 5-10 μg/ml per 1 million cells/ml.
Immunoprecipitation:
Western Blotting:
Immunohistochemistry (frozen sections): Recommended dilution:4-8 μg/ml Positive tissue:tonsil
Immunocytochemistry:
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from cell culture supernatant by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 999 Human CDH1

Swiss-port # P12830 Human Cadherin-1

Gene Symbol CDH1
Gene Full Name cadherin 1, type 1, E-cadherin (epithelial)
Background CD324 / E-cadherin is an epithelial cell surface molecule, which provides calcium-dependent homophilic interactions with E-cadherin of another cell. These intaractions take part in morphogenetic programs controlling the maintenance of the structural and functional integrity of epithelia and affect invasive potential of epithelial neoplasms. CD324 / E-cadherin is implicated in cell growth and differentiation, cell recognition, and sorting during developmental morphogenesis, as well as in aggregation-dependent cell survival. CD324 / E-cadherin-mediated cell adhesion system is highly regulated from inside the cell by a number of intracellular signaling pathways.
Function Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.
E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production. [UniProt]
Research Area Cancer antibody; Developmental Biology antibody; Signaling Transduction antibody
PTM During apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3 to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm. The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway. Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system. The gamma-secretase-mediated cleavage promotes disassembly of adherens junctions.
N-glycosylation at Asn-637 is essential for expression, folding and trafficking.
Ubiquitinated by a SCF complex containing SKP2, which requires prior phosphorylation by CK1/CSNK1A1. Ubiquitinated by CBLL1/HAKAI, requires prior phosphorylation at Tyr-754.

Images (2) Click the Picture to Zoom In

  • ARG65402 anti-E Cadherin antibody [67A4] WB image

    Western blot: 20 µg of MCF7 cell lysate stained with ARG65402 anti-E Cadherin antibody [67A4] at 1:1000 dilution.

  • ARG65402 anti-E Cadherin antibody [67A4] WB image

    Western blot: 20 µg of MCF7 and MDA-MB-231 cell lysates stained with ARG65402 anti-E Cadherin antibody [67A4] at 1:500 dilution.

    MDA-MB-231 was a negative control.

Customer's Feedback

nuts_pic      Excellent

Review for anti-E Cadherin antibody [67A4]

Application:WB

Sample:MCF7

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

show all review

nuts_pic      Average

Review for anti-E Cadherin antibody [67A4]

Application:WB

Sample:MCF7 and MDA-MB-231 (negative control)

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

show all review

nuts_pic      Good

Review for anti-E Cadherin antibody [67A4]

Application:WB

Sample:MCF7

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

show all review

Clone References

A complex 3D human tissue culture system based on mammary stromal cells and silk scaffolds for modeling breast morphogenesis and function.

ICC/IF / Human

Wang X et al.
Biomaterials.,  (2010)

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Molecular events associated with epithelial to mesenchymal transition of nasopharyngeal carcinoma cells in the absence of Epstein-Barr virus genome.

Lin JC et al.
J Biomed Sci.,  (2009)

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Differential effects of allergens and irritants on early differentiating monocyte-derived dendritic cells.

Furio L et al.
Eur J Dermatol.,  (2008)

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Chronic renal allograft dysfunction: the role of T cell-mediated tubular epithelial to mesenchymal cell transition.

Robertson H et al.
J Am Soc Nephrol.,  (2004)

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