ARG65402

anti-E Cadherin antibody [67A4]

anti-E Cadherin antibody [67A4] for Flow cytometry,ICC/IF,IHC-Frozen sections,Immunoprecipitation,Western blot and Human

EMT Study antibody; Epithelial Marker antibody
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Overview

Product Description Mouse Monoclonal antibody [67A4] recognizes E Cadherin
Tested Reactivity Hu
Tested Application FACS, ICC/IF, IHC-Fr, IP, WB
Specificity The mouse monoclonal antibody 67A4 recognizes CD324 / Ecadherin, an approximately 100 kDa epithelial cell adhesion molecule, whose detection is important for determination of invasive potential of epithelial neoplasms.
HLDA VIII
Host Mouse
Clonality Monoclonal
Clone 67A4
Isotype IgG1
Target Name E Cadherin
Immunogen T-47D cells_x000D_
Conjugation Un-conjugated
Full Name cadherin 1, type 1, E-cadherin (epithelial)
Alternate Names Uvomorulin; Arc-1; Cadherin-1; E-cadherin; CDHE; CD antigen CD324; ECAD; CAM 120/80; LCAM; Epithelial cadherin; UVO; CD324

Application Instructions

Application Suggestion
Tested Application Dilution
FACS5 - 10 µg/10^6 cells
ICC/IFAssay-dependent
IHC-Fr4 - 8 µg/ml
IPAssay-dependent
WB1 - 4 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control FACS: CACO-2 and HT-29
IHC-Fr: Tonsil
Calculated MW 97 kDa

Properties

Form Liquid
Purification Purified from cell culture supernatant by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 999 Human CDH1

Swiss-port # P12830 Human Cadherin-1

Gene Symbol CDH1
Gene Full Name cadherin 1, type 1, E-cadherin (epithelial)
Background E Cadherin is a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16. [provided by RefSeq, Nov 2015]
Function Cadherins are calcium-dependent cell adhesion proteins (PubMed:11976333). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed:11976333). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.

E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.

(Microbial infection) Serves as a receptor for Listeria monocytogenes; internalin A (InlA) binds to this protein and promotes uptake of the bacteria. [UniProt]
Research Area EMT Study antibody; Epithelial Marker antibody
PTM During apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3 to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm. The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway. Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system. The gamma-secretase-mediated cleavage promotes disassembly of adherens junctions.
N-glycosylation at Asn-637 is essential for expression, folding and trafficking.
Ubiquitinated by a SCF complex containing SKP2, which requires prior phosphorylation by CK1/CSNK1A1. Ubiquitinated by CBLL1/HAKAI, requires prior phosphorylation at Tyr-754.

Images (2) Click the Picture to Zoom In

  • ARG65402 anti-E Cadherin antibody [67A4] WB image

    Western blot: 20 µg of MCF7 cell lysate stained with ARG65402 anti-E Cadherin antibody [67A4] at 1:1000 dilution.

  • ARG65402 anti-E Cadherin antibody [67A4] WB image

    Western blot: 20 µg of MCF7 and MDA-MB-231 cell lysates stained with ARG65402 anti-E Cadherin antibody [67A4] at 1:500 dilution.

    MDA-MB-231 was a negative control.

Customer's Feedback

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Review for anti-E Cadherin antibody [67A4]

Application:WB

Sample:MCF7

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Average

Review for anti-E Cadherin antibody [67A4]

Application:WB

Sample:MCF7 and MDA-MB-231 (negative control)

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Good

Review for anti-E Cadherin antibody [67A4]

Application:WB

Sample:MCF7

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Clone References

A complex 3D human tissue culture system based on mammary stromal cells and silk scaffolds for modeling breast morphogenesis and function.

ICC/IF / Human

Wang X et al.
Biomaterials.,  (2010)

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Molecular events associated with epithelial to mesenchymal transition of nasopharyngeal carcinoma cells in the absence of Epstein-Barr virus genome.

Lin JC et al.
J Biomed Sci.,  (2009)

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Differential effects of allergens and irritants on early differentiating monocyte-derived dendritic cells.

Furio L et al.
Eur J Dermatol.,  (2008)

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Chronic renal allograft dysfunction: the role of T cell-mediated tubular epithelial to mesenchymal cell transition.

Robertson H et al.
J Am Soc Nephrol.,  (2004)

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