ARG66195

anti-E Cadherin antibody [SQab1717]

anti-E Cadherin antibody [SQab1717] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

EMT Study antibody; Epithelial Marker antibody
nuts_pic
1
publication_link Publication3

Overview

Product Description Recombinant Rabbit Monoclonal antibody [SQab1717] recognizes E Cadherin
Tested Reactivity Hu
Tested Application IHC-P, WB
Host Rabbit
Clonality Monoclonal
Clone SQab1717
Target Name E Cadherin
Antigen Species Human
Immunogen Synthetic peptide around aa. 600‐700 of E‐Cadherin.
Conjugation Un-conjugated
Alternate Names Uvomorulin; Arc-1; Cadherin-1; E-cadherin; CDHE; CD antigen CD324; ECAD; CAM 120/80; LCAM; Epithelial cadherin; UVO; CD324

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50
WB1:1000  ‐ 1:5000
Application Note IHC: Antigen retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 999 Human CDH1

Swiss-port # P12830 Human Cadherin-1

Gene Symbol CDH1
Gene Full Name cadherin 1, type 1
Background E Cadherin is a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16. [provided by RefSeq, Nov 2015]
Function Cadherins are calcium-dependent cell adhesion proteins (PubMed:11976333). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed:11976333). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.

E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.

(Microbial infection) Serves as a receptor for Listeria monocytogenes; internalin A (InlA) binds to this protein and promotes uptake of the bacteria. [UniProt]
Highlight Related Antibody Duos and Panels:
ARG30320 EMT Marker Antibody Panel
Related products:
E Cadherin antibodies; E Cadherin ELISA Kits; E Cadherin Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Related news:
Cancer Pathology Markers (SQ clones)
New EMT antibody panel is released
Research Area EMT Study antibody; Epithelial Marker antibody
Calculated MW 97 kDa
PTM During apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3 to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm. The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway. Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system. The gamma-secretase-mediated cleavage promotes disassembly of adherens junctions.
N-glycosylation at Asn-637 is essential for expression, folding and trafficking.
Ubiquitinated by a SCF complex containing SKP2, which requires prior phosphorylation by CK1/CSNK1A1. Ubiquitinated by CBLL1/HAKAI, requires prior phosphorylation at Tyr-754.

Images (3) Click the Picture to Zoom In

  • ARG66195 anti-E Cadherin antibody [SQab1717] WB image

    Western blot: 20 µg of MCF7 cell lysate stained with ARG66195 anti-E Cadherin antibody [SQab1717] at 1:2000 dilution.

  • ARG66195 anti-E Cadherin antibody [SQab1717] IHC-P image

    Immunohistochemistry: Formalin‐fixed and paraffin‐embedded Human skin tissue stained with ARG66195 anti-E Cadherin antibody [SQab1717] at 1:50 dilution.

    Antigen retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0

  • ARG66195 anti-E Cadherin antibody [SQab1717] IHC-P image

    Immunohistochemistry: Formalin‐fixed and paraffin‐embedded Human gastric adenocarcinoma tissue stained with ARG66195 anti-E Cadherin antibody [SQab1717] at 1:50 dilution.

    Antigen retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0

Customer's Feedback

nuts_pic      Excellent

Review for anti-E Cadherin antibody [SQab1717]

Application:WB

Sample:MCF7

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:2000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Specific References

Anticancer Effects and Molecular Mechanisms of Apigenin in Cervical Cancer Cells

WB, IHC-P / Human

Ya-Hui Chen et al.
Cancers (Basel).,  (2022)

publication_link

 

hr_line

A novel mechanism driving poor-prognostic gastric cancer: overexpression of the transcription factor Krüppel-like factor 16 promotes growth and metastasis of gastric cancer through regulating the Notch pathway.

WB / Human

Ding-Ping Sun et al.
Am J Cancer Res.,  (2021)

publication_link

 

hr_line

Tumor-associated neutrophils induce EMT by IL-17a to promote migration and invasion in gastric cancer cells.

WB / Human

Li Sen et al.
J Exp Clin Cancer Res.,  (2019)

publication_link

 

hr_line