ARG45526
anti-E2F3 antibody
anti-E2F3 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes E2F3 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | E2F3 |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human E2F3. |
| Conjugation | Un-conjugated |
| Alternate Names | E2F3; E2F Transcription Factor 3; Transcription Factor E2F3; E2F-3; KIAA0075 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Observed Size | 45 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | E2F3 |
| Gene Full Name | E2F Transcription Factor 3 |
| Background | This gene encodes a member of a small family of transcription factors that function through binding of DP interaction partner proteins. The encoded protein recognizes a specific sequence motif in DNA and interacts directly with the retinoblastoma protein (pRB) to regulate the expression of genes involved in the cell cycle. Altered copy number and activity of this gene have been observed in a number of human cancers. There are pseudogenes for this gene on chromosomes 2 and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013] |
| Function | Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F3 binds specifically to RB1 in a cell-cycle dependent manner. Inhibits adipogenesis, probably through the repression of CEBPA binding to its target gene promoters. [UniProt] |
| Cellular Localization | Nucleus. [UniProt] |
| Calculated MW | 49 kDa |
Images (5) Click the Picture to Zoom In
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ARG45526 anti-E2F3 antibody IHC-P image
Immunohistochemistry: Human liver cancer stained with ARG45526 anti-E2F3 antibody at 2 μg/ml dilution.
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ARG45526 anti-E2F3 antibody WB image
Western blot: 293T stained with ARG45526 anti-E2F3 antibody at 0.5 μg/ml dilution.
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ARG45526 anti-E2F3 antibody FACS image
Flow Cytometry: HL-60 stained with ARG45526 anti-E2F3 antibody at 1 µg/10^6 cells dilution.
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ARG45526 anti-E2F3 antibody WB image
Western blot: Rat lung stained with ARG45526 anti-E2F3 antibody at 0.5 μg/ml dilution.
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ARG45526 anti-E2F3 antibody WB image
Western blot: Mouse spleen stained with ARG45526 anti-E2F3 antibody at 0.5 μg/ml dilution.
