ARG45732
anti-ECSIT antibody
anti-ECSIT antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes ECSIT |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ECSIT |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human ECSIT. |
| Conjugation | Un-conjugated |
| Alternate Names | ECSIT; ECSIT Signaling Integrator; SITPEC; Evolutionarily Conserved Signaling Intermediate In Toll Pathway, Mitochondrial; ECSIT Signalling Integrator; Likely Ortholog Of Mouse Signaling Intermediate In Toll Pathway Evolutionarily Conserved; Signaling Intermediate In Toll Pathway Evolutionarily Conserved Ortholog (Mouse); ECSIT Homolog (Drosophila); Protein SITPEC; ECSIT Homolog |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Observed Size | 49 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9BQ95 Human Evolutionarily conserved signaling intermediate in Toll pathway, mitochond |
|---|---|
| Gene Symbol | ECSIT |
| Gene Full Name | ECSIT Signaling Integrator |
| Background | Enables molecular adaptor activity. Involved in regulation of oxidoreductase activity; regulation of protein complex stability; and toll-like receptor 4 signaling pathway. Located in cytosol; mitochondrion; and nucleoplasm. [provided by Alliance of Genome Resources, Feb 2025] |
| Function | UniProtKB/Swiss-Prot Summary for ECSIT Gene Adapter protein that plays a role in different signaling pathways including TLRs and IL-1 pathways or innate antiviral induction signaling. Plays a role in the activation of NF-kappa-B by forming a signal complex with TRAF6 and TAK1/MAP3K7 to activate TAK1/MAP3K7 leading to activation of IKKs. [UniProt] |
| Cellular Localization | Cytoplasm; Mitochondrion; Nucleus. [UniProt] |
| Calculated MW | 49 kDa |
| PTM | Isopeptide bond; Ubl conjugation. [UniProt] |
Images (4) Click the Picture to Zoom In
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ARG45732 anti-ECSIT antibody IHC-P image
Immunohistochemistry: Human bladder adenosquamous carcinoma stained with ARG45732 anti-ECSIT antibody at 2 μg/ml dilution.
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ARG45732 anti-ECSIT antibody ICC/IF image
Immunofluorescence: PC-3 stained with ARG45732 anti-ECSIT antibody at 5 μg/ml dilution.
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ARG45732 anti-ECSIT antibody WB image
Western blot: PC-3 stained with ARG45732 anti-ECSIT antibody at 0.5 μg/ml dilution.
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ARG45732 anti-ECSIT antibody FACS image
Flow Cytometry: HepG2 stained with ARG45732 anti-ECSIT antibody at 1 µg/10^6 cells dilution.
