anti-EGF antibody [S-134]

anti-EGF antibody [S-134] for ELISA and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Signaling Transduction antibody


Product Description Mouse Monoclonal antibody [S-134] recognizes Human EGF
Tested Reactivity Hu
Tested Application ELISA
Specificity Does not cross react with other human cytokines tested such as IL-1β, IL-8, MCAF, TGF-β and SAA.
Host Mouse
Clonality Monoclonal
Clone S-134
Isotype IgG1, kappa
Target Name EGF
Antigen Species Human
Immunogen Purified recombinant human EGF, 6kDa, 53 amino acid residues
Conjugation Un-conjugated
Alternate Names Urogastrone; Pro-epidermal growth factor; URG; HOMG4; EGF

Application Instructions

Application Note ELISA: The antibody can be used as capture antibody in sandwich ELISA for human EGF detection in combination with EGF tracer (Cat. No.: ARG10200, clone S-146).

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Protein G affinity purified
Buffer 0.01M PBS (pH 7.2)
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 1950 Human EGF

Swiss-port # P01133 Human Pro-epidermal growth factor

Gene Symbol EGF
Gene Full Name epidermal growth factor
Background Epidermal growth factor (EGF), along with its receptor EGFR, plays an important role in cell proliferation, differentiation and survival. The binding of EGF to EGFR triggers the intrinsic tyrosine kinase activity of EGFR, activates signalling molecules and stimulates the synthesis of proteins and DNAs. EGF is crucial for maintaining mucosal lining in gastrointestinal duct and for healing of oral and gastro esophageal ulcers. EGF signalling is needed for cancer growth. Monoclonal antibodies that block EGFR have been applied for lung cancer and colon inhibition.
Function EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941). [UniProt]
Highlight Related Antibody Duos and Panels:
ARG30073 EGF ELISA Antibody Duo
Related products:
EGF antibodies; EGF ELISA Kits; EGF Duos / Panels; Anti-Mouse IgG secondary antibodies;
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Signaling Transduction antibody
Calculated MW 134 kDa
PTM O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).