ARG10013

anti-EGF antibody [S-21]

anti-EGF antibody [S-21] for ELISA and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Signaling Transduction antibody

Overview

Product Description Mouse Monoclonal antibody [S-21] recognizes EGF
Tested Reactivity Hu
Tested Application ELISA
Specificity Does not cross react with other human cytokines tested such as IL-1β, IL-8, MCAF, TGF-β and SAA.
Host Mouse
Clonality Monoclonal
Clone S-21
Isotype IgG1,k
Target Name EGF
Antigen Species Human
Immunogen Purified recombinant human EGF, 6kDa, 53 amino acid residues
Conjugation Un-conjugated
Full Name epidermal growth factor
Alternate Names Urogastrone; Pro-epidermal growth factor; URG; HOMG4; EGF

Application Instructions

Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Protein G affinity purified
Buffer 0.01M PBS (pH 7.2)
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 1950 Human EGF

Swiss-port # P01133 Human Pro-epidermal growth factor

Gene Symbol EGF
Gene Full Name epidermal growth factor
Background This gene encodes a member of the epidermal growth factor superfamily. The encoded protein is synthesized as a large precursor molecule that is proteolytically cleaved to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding the high affinity cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010]
Function EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941). [UniProt]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Signaling Transduction antibody
PTM O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).