ARG10518

anti-EGFR antibody [EGFR1]

anti-EGFR antibody [EGFR1] for Flow cytometry,ICC/IF,IHC-Frozen sections,Immunoprecipitation,Western blot and Horse,Human,Mouse

Cancer antibody; Signaling Transduction antibody

Overview

Product Description

Mouse Monoclonal antibody [EGFR1] recognizes EGFR

Tested Reactivity Hu, Ms, Hrs
Tested Application FACS, ICC/IF, IHC-Fr, IP, WB
Host Mouse
Clonality Monoclonal
Clone EGFR1
Isotype IgG2b
Target Name EGFR
Antigen Species Human
Immunogen Human epidermoid carcinoma line A431
Conjugation Un-conjugated
Full Name epidermal growth factor receptor
Alternate Names PIG61; ERBB1; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; NISBD2; Epidermal growth factor receptor; ERBB; HER1; EC 2.7.10.1; mENA

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1µg for 10^6  cells. (unsuitable for fixed cells)
ICC/IF1 µg/ml
IHC-FrAssay-dependent.
IPAssay-dependent.
WBAssay-dependent.
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 175 kDa

Properties

Form Liquid
Purification Purified by affinity chromatography.
Buffer PBS and 0.02% Sodium azide
Preservative 0.02% Sodium azide
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 13649 Mouse EGFR

GeneID: 1956 Human EGFR

Swiss-port # P00533 Human Epidermal growth factor receptor

Swiss-port # Q01279 Mouse Epidermal growth factor receptor

Gene Symbol EGFR
Gene Full Name epidermal growth factor receptor
Background The EGFR family of type I growth factor receptor tyrosine kinases includes EGFR (HER1), c-erbB2 (HER2; neu), c-erbB3 (HER3) and c-erbB4 (HER4). Dysregulation of EGFR signaling as a consequence of overexpression, amplification and mutation of the EGFR gene occurs frequently in several types of cancers and many become dependent on EGFR signaling to maintain their malignant phenotypes.
Function Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin. Isoform 2 may act as an antagonist of EGF action. [Uniprot]
Research Area Cancer antibody; Signaling Transduction antibody
PTM Phosphorylation at Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Phosphorylation at Thr-678 and Thr-693 by PRKD1 inhibits EGF-induced MAPK8/JNK1 activation. Dephosphorylation by PTPRJ prevents endocytosis and stabilizes the receptor at the plasma membrane. Autophosphorylation at Tyr-1197 is stimulated by methylation at Arg-1199 and enhances interaction with PTPN6. Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3. Dephosphorylated by PTPN1 and PTPN2.
Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occurs. Deubiquitination by OTUD7B prevents degradation. Ubiquitinated by RNF115 and RNF126 (By similarity).
Methylated. Methylation at Arg-1199 by PRMT5 stimulates phosphorylation at Tyr-1197.

Clone References

Effect of cycloheximide on epidermal growth factor receptor trafficking and signaling.

WB / Human

Oksvold MP et al.
FEBS Lett.,  (2012)

publication_link

 

hr_line

Tumor penetration and epidermal growth factor receptor saturation by panitumumab correlate with antitumor activity in a preclinical model of human cancer.

IP / Human

Freeman DJ et al.
Mol Cancer.,  (2012)

publication_link

 

hr_line

Sperm epidermal growth factor receptor (EGFR) mediates α7 acetylcholine receptor (AChR) activation to promote fertilization.

IP / 

Jaldety Y et al.
J Biol Chem.,  (2012)

publication_link

 

hr_line

Optical nanomanipulations of malignant cells: controlled cell damage and fusion.

BL / Human

Minai L et al.
Small.,  (2012)

publication_link

 

hr_line

Bispecific designed ankyrin repeat proteins (DARPins) targeting epidermal growth factor receptor inhibit A431 cell proliferation and receptor recycling.

FACS / Human

Boersma YL et al.
J Biol Chem.,  (2011)

publication_link

 

hr_line

Expression of epidermal growth factor receptor or ErbB3 facilitates geldanamycin-induced down-regulation of ErbB2.

FACS / Human

Pedersen NM et al.
Mol Cancer Res.,  (2009)

publication_link

 

hr_line

Direct interaction of Cbl with pTyr 1045 of the EGF receptor (EGFR) is required to sort the EGFR to lysosomes for degradation.

WB / Pig

Grøvdal LM et al.
Exp Cell Res.,  (2004)

publication_link

 

hr_line