anti-EGFR antibody [SQab1726]

anti-EGFR antibody [SQab1726] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat

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Product Description

Recombinant Rabbit Monoclonal antibody [SQab1726] recognizes EGFR

Tested Reactivity Hu, Ms, Rat
Tested Application FACS, ICC/IF, IHC-P, IP, WB
Host Rabbit
Clonality Monoclonal
Clone SQab1726
Target Name EGFR
Antigen Species Human
Immunogen Synthetic peptide around the C-terminus of EGFR.
Conjugation Un-conjugated
Alternate Names PIG61; ERBB1; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; NISBD2; Epidermal growth factor receptor; ERBB; HER1; EC; mENA

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:50 - 1:200
ICC/IF1:200 - 1:800
IHC-P1:100 - 1:200
WB1:5000 - 10000
Application Note IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 13649 Mouse EGFR

GeneID: 1956 Human EGFR

Swiss-port # P00533 Human Epidermal growth factor receptor

Swiss-port # Q01279 Mouse Epidermal growth factor receptor

Gene Symbol EGFR
Gene Full Name epidermal growth factor receptor
Background EGFR is a transmembrane glycoprotein. It is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016]
Function EGFR: Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses (PubMed:2790960, PubMed:10805725, PubMed:27153536). Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF (PubMed:2790960, PubMed:7679104, PubMed:8144591, PubMed:9419975, PubMed:15611079, PubMed:12297049, PubMed:27153536, PubMed:20837704). Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules (PubMed:27153536). May also activate the NF-kappa-B signaling cascade (PubMed:11116146). Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling (PubMed:11602604). Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin (PubMed:11483589). Plays a role in enhancing learning and memory performance.

Isoform 2 may act as an antagonist of EGF action.

(Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes and facilitates its cell entry. Mediates HCV entry by promoting the formation of the CD81-CLDN1 receptor complexes that are essential for HCV entry and by enhancing membrane fusion of cells expressing HCV envelope glycoproteins. [UniProt]
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Calculated MW 134 kDa
PTM Phosphorylation at Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Phosphorylation at Thr-678 and Thr-693 by PRKD1 inhibits EGF-induced MAPK8/JNK1 activation. Dephosphorylation by PTPRJ prevents endocytosis and stabilizes the receptor at the plasma membrane. Autophosphorylation at Tyr-1197 is stimulated by methylation at Arg-1199 and enhances interaction with PTPN6. Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3. Dephosphorylated by PTPN1 and PTPN2.
Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occurs. Deubiquitination by OTUD7B prevents degradation. Ubiquitinated by RNF115 and RNF126 (By similarity).
Methylated. Methylation at Arg-1199 by PRMT5 stimulates phosphorylation at Tyr-1197.

Images (6) Click the Picture to Zoom In

  • ARG66204 anti-EGFR antibody [SQab1726] WB image

    Western blot: 30 µg of A549 and 293T cell lysates stained with ARG66204 anti-EGFR antibody [SQab1726] at 1:2000 dilution.

  • ARG66204 anti-EGFR antibody [SQab1726] ICC/IF image

    Immunofluorescence: A431 cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for 30 min at RT. Cells were stained with ARG66204 anti-EGFR antibody [SQab1726] (green) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

  • ARG66204 anti-EGFR antibody [SQab1726] FACS image

    Flow Cytometry: A431 cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were stained with ARG66204 anti-EGFR antibody [SQab1726] (red) at 1:200 dilution in 1x PBS/1% BSA for 30 min at RT, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control.

  • ARG66204 anti-EGFR antibody [SQab1726] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human lung cancer tissue stained with ARG66204 anti-EGFR antibody [SQab1726] at 1:200 dilution. Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0.

  • ARG66204 anti-EGFR antibody [SQab1726] WB image

    Western blot: 10 µg of PC-3, SH-SY5Y, A549, HeLa, A431, HaCat, Mouse skin and Rat skin lysates stained with ARG66204 anti-EGFR antibody [SQab1726] at 1:5000 dilution.

  • ARG66204 anti-EGFR antibody [SQab1726] IP image

    Immunoprecipitation: 0.4 mg of A431 whole cell lysate immunoprecipitated (1:10) and stained with ARG66204 anti-EGFR antibody [SQab1726]. 1) ARG66204 IP in A431 whole cell lysate, 2) PBS instead of ARG66204 in A431 whole cell lysate, and 3) A431 whole cell lysate, 10 µg (input).

Customer's Feedback

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Review for anti-EGFR antibody [SQab1726]


Sample:A549 and 293T

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:2000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Specific References

16-Hydroxycleroda-3,13-dien-15,16-olide Induces Apoptosis in Human Bladder Cancer Cells through Cell Cycle Arrest, Mitochondria ROS Overproduction, and Inactivation of EGFR-Related Signalling Pathways.

WB / Human

Yu-Chi Chen et al.
Molecules.,  (2020)