ARG45557
anti-EHD3 antibody
anti-EHD3 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Monkey
Overview
| Product Description | Rabbit Polyclonal antibody recognizes EHD3 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Mk |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | EHD3 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to C-terminal region of human EHD3. |
| Conjugation | Un-conjugated |
| Alternate Names | EHD3; EH Domain Containing 3; PAST3; EH Domain-Containing Protein 3; PAST Homolog 3; EHD2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Observed Size | 70 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | EHD3 |
| Gene Full Name | EH Domain Containing 3 |
| Background | Predicted to enable nucleic acid binding activity. Involved in several processes, including cytosolic transport; endocytic recycling; and protein homooligomerization. Acts upstream of or within protein localization to plasma membrane and regulation of cardiac muscle cell membrane potential. Located in ciliary pocket membrane and recycling endosome membrane. [provided by Alliance of Genome Resources, Feb 2025] |
| Function | TP- and membrane-binding protein that controls membrane reorganization/tubulation upon ATP hydrolysis. In vitro causes tubulation of endocytic membranes. Binding to phosphatidic acid induces its membrane tubulation activity (By similarity). Plays a role in endocytic transport. Involved in early endosome to recycling endosome compartment (ERC), retrograde early endosome to Golgi, and endosome to plasma membrane (rapid recycling) protein transport. Involved in the regulation of Golgi maintenance and morphology. [UniProt] |
| Cellular Localization | Cell membrane; Cell projection; Cilium; Endosome; Membrane. [UniProt] |
| Calculated MW | 61 kDa |
| PTM | Acetylation; Isopeptide bond; Phosphoprotein; Ubl conjugation. [UniProt] |
Images (6) Click the Picture to Zoom In
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ARG45557 anti-EHD3 antibody IHC-P image
Immunohistochemistry: Human ovarian serous adenocarcinoma stained with ARG45557 anti-EHD3 antibody at 2 μg/ml dilution.
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ARG45557 anti-EHD3 antibody ICC/IF image
Immunofluorescence: MCF-7 stained with ARG45557 anti-EHD3 antibody at 5 μg/ml dilution.
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ARG45557 anti-EHD3 antibody WB image
Western blot: A549 stained with ARG45557 anti-EHD3 antibody at 0.5 μg/ml dilution.
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ARG45557 anti-EHD3 antibody FACS image
Flow Cytometry: MCF-7 stained with ARG45557 anti-EHD3 antibody at 1 µg/10^6 cells dilution.
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ARG45557 anti-EHD3 antibody IHC-P image
Immunohistochemistry: Rat brain stained with ARG45557 anti-EHD3 antibody at 2 μg/ml dilution.
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ARG45557 anti-EHD3 antibody WB image
Western blot: monkey heart stained with ARG45557 anti-EHD3 antibody at 0.5 μg/ml dilution.
