anti-ELK1 phospho (Ser383) antibody
anti-ELK1 phospho (Ser383) antibody for Western blot and Rat
Cancer antibody; Gene Regulation antibody; Signaling Transduction antibody
|Product Description||Rabbit Polyclonal antibody recognizes ELK1 phospho (Ser383)|
|Predict Reactivity||Hu, Ms, Dog, NHuPrm, Zfsh|
|Immunogen||Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser383 conjugated to KLH|
|Alternate Names||ETS domain-containing protein Elk-1|
|Application Note||Specific for ~46k Elk-1 phosphorylated at Ser383. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|Buffer||10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol|
|Stabilizer||0.1 mg/ml BSA, 50% Glycerol|
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||ELK1, member of ETS oncogene family|
|Background||Elk-1 is a transcription factor involved in mediating gene transcription in response to growth factors (Hill and Treisman, 1995). Elk-1 is thought to be phosphorylated by MAP kinase at Ser383 and phosphorylation at this site is essential for the transcriptional activity of Elk-1 (Li et al., 2003). Phosphorylation of Elk-1 has also been implicated in synaptic plasticity in the adult hippocampus (Thiels et al., 2002).|
|Research Area||Cancer antibody; Gene Regulation antibody; Signaling Transduction antibody|
|Calculated MW||45 kDa|
|PTM||Sumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention.
On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Also phosphorylated on Ser-383 by MAPK8 and/or MAKP9. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity. Phosphorylated and activated by CAMK4, MAPK11, MAPK12 and MAPK14. Upon bFGF stimulus, phosphorylated by PAK1 (By similarity).
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