ARG22201

anti-ENaC beta antibody

anti-ENaC beta antibody for ICC/IF,Immunohistochemistry,Immunoprecipitation,Western blot and Hamster,Human,Mouse,Rat,Xenopus laevis

Overview

Product Description Rabbit Polyclonal antibody recognizes ENaC beta
Tested Reactivity Hu, Ms, Rat, Hm, Xenopus laevis
Tested Application ICC/IF, IHC, IP, WB
Specificity Detects ~87kDa.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name ENaC beta
Antigen Species Rat
Immunogen Synthetic peptide around aa. 617-638 (C-terminus) of Rat ENaC Beta
Conjugation Un-conjugated
Alternate Names Beta-ENaC; Nonvoltage-gated sodium channel 1 subunit beta; Epithelial Na; ENaCb; Amiloride-sensitive sodium channel subunit beta; ENaCB; Beta-NaCH; SCNEB; BESC1; ENaCbeta

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IFAssay-dependent
IHC1:100
IPAssay-dependent
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.09% Sodium azide and 50% Glycerol
Preservative 0.09% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 20277 Mouse SCNN1B

GeneID: 24767 Rat SCNN1B

GeneID: 6338 Human SCNN1B

Gene Symbol Scnn1b
Gene Full Name sodium channel, non-voltage-gated 1, beta subunit
Background Nonvoltage-gated, amiloride-sensitive, sodium channels control fluid and electrolyte transport across epithelia in many organs. These channels are heteromeric complexes consisting of 3 subunits: alpha, beta, and gamma. This gene encodes the beta subunit, and mutations in this gene have been associated with pseudohypoaldosteronism type 1 (PHA1), and Liddle syndrome. [provided by RefSeq, Apr 2009]
Function Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception. [UniProt]
Cellular Localization Apical cell membrane
Calculated MW 73 kDa
PTM Phosphorylated on serine and threonine residues. Aldosterone and insulin increase the basal level of phosphorylation.
N-glycosylated. N-glycosylation is required for interaction with BPIFA1.

Images (4) Click the Picture to Zoom In

  • ARG22201 anti-ENaC beta antibody IHC image

    Immunohistochemistry: Rat kidney tissue stained with ARG22201 anti-ENaC beta antibody.

  • ARG22201 anti-ENaC beta antibody WB image

    Western blot: Rat kidney tissue lysates stained with ARG22201 anti-ENaC beta antibody at 1:1000 dilution.

  • ARG22201 anti-ENaC beta antibody WB image

    Western blot: 1) Mouse mpkCCD cell lysate, and 2) FRT expressing tagged beta-mENaC stained with ARG22201 anti-ENaC beta antibody.

  • ARG22201 anti-ENaC beta antibody WB image

    Western blot: Mouse kidney cortex stained with ARG22201 anti-ENaC beta antibody at 1:1000 dilution. Low-salt diet (lanes 1-4) compared to a high-salt diet (lanes 5-8).