ARG51579

anti-ERBB2 / HER2 phospho (Tyr1221 / Tyr1222) antibody

anti-ERBB2 / HER2 phospho (Tyr1221 / Tyr1222) antibody for Western blot,ICC/IF and Human

Cancer antibody; Controls and Markers antibody; Signaling Transduction antibody; Circulating Tumor Cells BioMarker antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes ERBB2 / HER2 phospho (Tyr1221 / Tyr1222)
Tested Reactivity Hu
Tested Application ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name ERBB2 / HER2
Antigen Species Human
Immunogen Peptide sequence around phosphorylation site of tyrosine1221/1222 (N-L-Y(p)-Y(p)-W) derived from Human HER2.
Conjugation Un-conjugated
Alternate Names p185erbB2; Proto-oncogene c-ErbB-2; Metastatic lymph node gene 19 protein; Proto-oncogene Neu; NGL; EC 2.7.10.1; CD340; CD antigen CD340; TKR1; HER-2; Tyrosine kinase-type cell surface receptor HER2; HER2; NEU; HER-2/neu; MLN 19; Receptor tyrosine-protein kinase erbB-2

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:100 - 1:200
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Buffer PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2064 Human ERBB2

Swiss-port # P04626 Human Receptor tyrosine-protein kinase erbB-2

Gene Symbol ERBB2
Gene Full Name erb-b2 receptor tyrosine kinase 2
Background Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Not activated by EGF, TGF-alpha and amphiregulin.
Function Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth. [UniProt]
Highlight Related Antibody Duos and Panels:
ARG30034 Phospho ERBB2 / HER2 Antibody Duo (Total, pY1221/1222)
Related products:
ERBB2 antibodies; ERBB2 ELISA Kits; ERBB2 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Research Area Cancer antibody; Controls and Markers antibody; Signaling Transduction antibody; Circulating Tumor Cells BioMarker antibody
Calculated MW 138 kDa
PTM Autophosphorylated. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit (Probable). Ligand-binding increases phosphorylation on tyrosine residues (PubMed:27134172). Signaling via SEMA4C promotes phosphorylation at Tyr-1248 (PubMed:17554007). Dephosphorylated by PTPN12 (PubMed:27134172).

Images (3) Click the Picture to Zoom In

  • ARG51579 anti-ERBB2 / HER2 phospho (Tyr1221 / Tyr1222) antibody WB image

    Western blot: Extracts from MDA cells untreated or treated with EGF stained with ARG51579 anti-ERBB2 / HER2 phospho (Tyr1221 / Tyr1222) antibody.

  • ARG51579 anti-ERBB2 / HER2 phospho (Tyr1221 / Tyr1222) antibody WB image

    Western blot: Extracts from HeLa cells, treated with EGF or calf intestinal phosphatase (CIP), stained with ARG51579 anti-ERBB2 / HER2 phospho (Tyr1221 / Tyr1222) antibody.

  • ARG51579 anti-ERBB2 / HER2 phospho (Tyr1221 / Tyr1222) antibody ICC/IF image

    Immunofluorescence: methanol-fixed MCF cells stained with ARG51579 anti-ERBB2 / HER2 phospho (Tyr1221 / Tyr1222) antibody.