ARG51811

anti-ERBB3 / HER3 phospho (Tyr1328) antibody

anti-ERBB3 / HER3 phospho (Tyr1328) antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,ICC/IF and Human,Mouse,Rat

Cancer antibody; Controls and Markers antibody; Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes ERBB3 / HER3 phospho (Tyr1328)
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name ERBB3 / HER3
Antigen Species Human
Immunogen Peptide sequence around phosphorylation site of tyrosine 1328 (P-D-Y(p)-W-H) derived from Human Her3/ErbB3.
Conjugation Un-conjugated
Alternate Names MDA-BF-1; LCCS2; p180-ErbB3; Proto-oncogene-like protein c-ErbB-3; c-erbB3; p85-sErbB3; Tyrosine kinase-type cell surface receptor HER3; p45-sErbB3; erbB3-S; HER3; c-erbB-3; ErbB-3; EC 2.7.10.1; Receptor tyrosine-protein kinase erbB-3

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:100 - 1:200
IHC-P1:50 - 1:100
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Buffer PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 13867 Mouse ERBB3

GeneID: 2065 Human ERBB3

GeneID: 29496 Rat ERBB3

Gene Symbol ERBB3
Gene Full Name erb-b2 receptor tyrosine kinase 3
Background Binds and is activated by neuregulins and NTAK.
Function Binds and is activated by neuregulins and NTAK. May also be activated by CSPG5. [UniProt]
Research Area Cancer antibody; Controls and Markers antibody; Signaling Transduction antibody
Calculated MW 148 kDa
PTM Autophosphorylated (PubMed:20351256). Ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase (PubMed:20682778).

Images (3) Click the Picture to Zoom In

  • ARG51811 anti-ERBB3 / HER3 phospho (Tyr1328) antibody WB image

    Western blot: Extracts from A431 cells untreated or treated with EGF stained with ARG51811 anti-ERBB3 / HER3 phospho (Tyr1328) antibody.

  • ARG51811 anti-ERBB3 / HER3 phospho (Tyr1328) antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51811 anti-ERBB3 / HER3 phospho (Tyr1328) antibody (left) or the same antibody preincubated with blocking peptide (right).

  • ARG51811 anti-ERBB3 / HER3 phospho (Tyr1328) antibody ICC/IF image

    Immunofluorescence: methanol-fixed MCF7 cells stained with ARG51811 anti-ERBB3 / HER3 phospho (Tyr1328) antibody.