ARG45533

anti-ERM / Etv5 antibody

anti-ERM / Etv5 antibody for Flow cytometry,ICC/IF,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes ERM / Etv5
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name ERM / Etv5
Antigen Species Human
Immunogen Recombinant protein containing to human ERM / Etv5.
Conjugation Un-conjugated
Alternate Names ETV5; ETS Variant Transcription Factor 5; ERM; ETS Translocation Variant 5; Ets-Related Protein ERM; Ets-Related Molecule; Ets Variant Gene 5 (Ets-Related Molecule); ETS Variant 5

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 3 µg/10^6 cells
ICC/IF5 μg/ml
WB0.25-0.5 μg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 70 kDa

Properties

Form Liquid
Purification Affinity purified
Buffer 0.9% NaCl, 0.2% Na2HPO4, 0.01% Sodium azide and 4% Trehalose.
Preservative 0.01% Sodium azide
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 104156 Mouse ETV5

GeneID: 2119 Human ETV5

Swiss-port # P41161 Human ETS translocation variant 5

Swiss-port # Q9CXC9 Mouse ETS translocation variant 5

Gene Symbol ETV5
Gene Full Name ETS Variant Transcription Factor 5
Background Enables DNA-binding transcription activator activity, RNA polymerase II-specific and RNA polymerase II transcription regulatory region sequence-specific DNA binding activity. Involved in cellular response to oxidative stress; negative regulation of transcription by RNA polymerase II; and positive regulation of transcription by RNA polymerase II. Located in nucleoplasm. [provided by Alliance of Genome Resources, Feb 2025]
Function Binds to DNA sequences containing the consensus nucleotide core sequence 5'-GGAA.-3'. [UniProt]
Cellular Localization Nucleus. [UniProt]
Calculated MW 58 kDa
PTM Isopeptide bond; Phosphoprotein; Ubl conjugation. [UniProt]

Images (5) Click the Picture to Zoom In

  • ARG45533 anti-ERM / Etv5 antibody ICC/IF image

    Immunofluorescence: MCF-7 stained with ARG45533 anti-ERM / Etv5 antibody at 5 μg/ml dilution.

  • ARG45533 anti-ERM / Etv5 antibody WB image

    Western blot: A549 stained with ARG45533 anti-ERM / Etv5 antibody at 0.5 μg/ml dilution.

  • ARG45533 anti-ERM / Etv5 antibody FACS image

    Flow Cytometry: HL-60 stained with ARG45533 anti-ERM / Etv5 antibody at 1 µg/10^6 cells dilution.

  • ARG45533 anti-ERM / Etv5 antibody WB image

    Western blot: C6 stained with ARG45533 anti-ERM / Etv5 antibody at 0.5 μg/ml dilution.

  • ARG45533 anti-ERM / Etv5 antibody WB image

    Western blot: Neuro-2a stained with ARG45533 anti-ERM / Etv5 antibody at 0.5 μg/ml dilution.