ARG54703

anti-EZH2 antibody

anti-EZH2 antibody for Flow cytometry,ICC/IF,Immunohistochemistry,Western blot and Human,Mouse

Cancer antibody; Gene Regulation antibody; Polycomb Complexes antibody
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Overview

Product Description

Rabbit Polyclonal antibody recognizes EZH2

Tested Reactivity Hu, Ms
Tested Application FACS, ICC/IF, IHC, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name EZH2
Antigen Species Human
Immunogen Human EZH2 recombinant protein (NP_001190176.1).
Conjugation Un-conjugated
Alternate Names ENX-1; Lysine N-methyltransferase 6; KMT6; ENX1; WVS2; WVS; Enhancer of zeste homolog 2; EZH2b; Histone-lysine N-methyltransferase EZH2; EZH1; EC 2.1.1.43; KMT6A

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:10 - 1:50
ICC/IF1:10 - 1:50
IHC1:10 - 1:50
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Purification Protein A purified
Buffer PBS and 0.09% (W/V) Sodium azide
Preservative 0.09% (W/V) Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 14056 Mouse EZH2

GeneID: 2146 Human EZH2

Swiss-port # Q15910 Human Histone-lysine N-methyltransferase EZH2

Swiss-port # Q61188 Mouse Histone-lysine N-methyltransferase EZH2

Gene Symbol EZH2
Gene Full Name enhancer of zeste 2 polycomb repressive complex 2 subunit
Background This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Multiple alternatively splcied transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Feb 2011]
Function Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys- 27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4 and the nuclear receptor RORA. Regulates the circadian clock via histone methylation at the promoter of the circadian genes. Essential for the CRY1/2-mediated repression of the transcriptional activation of PER1/2 by the CLOCK-ARNTL/BMAL1 heterodimer; involved in the di and trimethylation of 'Lys-27' of histone H3 on PER1/2 promoters which is necessary for the CRY1/2 proteins to inhibit transcription. [From Uniprot]
Cellular Localization Nucleus
Highlight Related Antibody Duos and Panels:
ARG30309 Polycomb Complexes Antibody Panel (H3K27me3, EZH2, Ring1A, RNF2)
Related products:
EZH2 antibodies; EZH2 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Research Area Cancer antibody; Gene Regulation antibody; Polycomb Complexes antibody
Calculated MW 85 kDa
PTM Phosphorylated by AKT1. Phosphorylation by AKT1 reduces methyltransferase activity. Phosphorylation at Thr-345 by CDK1 and CDK2 promotes maintenance of H3K27me3 levels at EZH2-target loci, thus leading to epigenetic gene silencing.
Sumoylated.
Glycosylated: O-GlcNAcylation at Ser-75 by OGT increases stability of EZH2 and facilitates the formation of H3K27me3 by the PRC2/EED-EZH2 complex.

Images (5) Click the Picture to Zoom In

  • ARG54703 anti-EZH2 antibody WB image

    Western blot: 30 µg of U2OS, Jurkat and PC3 cell lysates stained with ARG54703 anti-EZH2 antibody at 1:1000 dilution

  • ARG54703 anti-EZH2 antibody WB image

    Western blot: 30 µg of SW480 and HCT116 cell lysates stained with ARG54703 anti-EZH2 antibody at 1:1000 dilution.

  • ARG54703 anti-EZH2 antibody ICC/IF image

    Immunofluorescence: 293 cells stained with ARG54703 anti-EZH2 antibody. Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).

  • ARG54703 anti-EZH2 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human testis tissue stained with ARG54703 anti-EZH2 antibody.

  • ARG54703 anti-EZH2 antibody FACS image

    Flow Cytometry: HeLa cells stained with ARG54703 anti-EZH2 antibody (right histogram) at 1:25 dilution or isotype control antibody (left histogram), followed by incubation with Alexa Fluor® 488 labelled secondary antibody.

Customer's Feedback

nuts_pic      Good

Review for anti-EZH2 antibody

Application:WB

Sample:SW480 and HCT116

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

Review for anti-EZH2 antibody

Application:WB

Sample:U2OS, Jurkat and PC3

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

Review for anti-EZH2 antibody

Application:WB

Sample:293T

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Good

Review for anti-EZH2 antibody

Application:WB

Sample:Jurkat

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC