ARG45731
anti-Elongin-C antibody
anti-Elongin-C antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Elongin-C |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Elongin-C |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human Elongin-C. |
| Conjugation | Un-conjugated |
| Alternate Names | ELOC; Elongin C; TCEB1; SIII; Transcription Elongation Factor B (SIII), Polypeptide 1 (15kDa, Elongin C); RNA Polymerase II Transcription Factor SIII Subunit C; Transcription Elongation Factor B Polypeptide 1; Transcription Elongation Factor B Subunit 1; Elongin 15 KDa Subunit; Elongin-C; SIII P15; Transcription Elongation Factor B (SIII), Polypeptide 1 (15kD, Elongin C); EloC |
Application Instructions
| Application Suggestion |
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Observed Size | 14 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ELOC |
| Gene Full Name | Elongin C |
| Background | This gene encodes the protein elongin C, which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation. Multiple alternatively spliced transcript variants encoding two distinct isoforms have been identified. [provided by RefSeq, Mar 2011] |
| Function | SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex). [UniProt] |
| Cellular Localization | Nucleus. [UniProt] |
| Calculated MW | 12 kDa |
| PTM | Ubl conjugation. [UniProt] |
Images (8) Click the Picture to Zoom In
-
ARG45731 anti-Elongin-C antibody IHC-P image
Immunohistochemistry: Human lung cancer stained with ARG45731 anti-Elongin-C antibody at 2 μg/ml dilution.
-
ARG45731 anti-Elongin-C antibody ICC/IF image
Immunofluorescence: PC-3 stained with ARG45731 anti-Elongin-C antibody at 5 μg/ml dilution.
-
ARG45731 anti-Elongin-C antibody WB image
Western blot: HepG2 stained with ARG45731 anti-Elongin-C antibody at 0.5 μg/ml dilution.
-
ARG45731 anti-Elongin-C antibody FACS image
Flow Cytometry: A549 stained with ARG45731 anti-Elongin-C antibody at 1 µg/10^6 cells dilution.
-
ARG45731 anti-Elongin-C antibody IHC-P image
Immunohistochemistry: Rat pancreas stained with ARG45731 anti-Elongin-C antibody at 2 μg/ml dilution.
-
ARG45731 anti-Elongin-C antibody WB image
Western blot: Rat brain stained with ARG45731 anti-Elongin-C antibody at 0.5 μg/ml dilution.
-
ARG45731 anti-Elongin-C antibody IHC-P image
Immunohistochemistry: Mouse pancreas stained with ARG45731 anti-Elongin-C antibody at 2 μg/ml dilution.
-
ARG45731 anti-Elongin-C antibody WB image
Western blot: Mouse brain stained with ARG45731 anti-Elongin-C antibody at 0.5 μg/ml dilution.
