ARG10517
anti-EpCAM antibody [AUA1]
anti-EpCAM antibody [AUA1] for ELISA,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Controls and Markers antibody; Epithelial Marker antibody; Circulating Tumor Cells BioMarker antibody
Overview
| Product Description | Mouse Monoclonal antibody [AUA1] recognizes EpCAM |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, FACS, ICC/IF, IHC-P, WB |
| Specificity | Cross reacts with breast carcinomas, kidney tubules, tonsil crypt epithelium and cells in some serious effusions. This is a good antibody for characterisation of all epithelial cells. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | AUA1 |
| Isotype | IgG1 |
| Target Name | EpCAM |
| Antigen Species | Human |
| Immunogen | LoVo cell line, a colon carcinoma cell line. |
| Conjugation | Un-conjugated |
| Full Name | epithelial cell adhesion molecule |
| Alternate Names | MIC18; EGP; Tumor-associated calcium signal transducer 1; Epithelial glycoprotein 314; KSA; Ep-CAM; Epithelial cell surface antigen; Adenocarcinoma-associated antigen; HNPCC8; Cell surface glycoprotein Trop-1; EGP40; TACSTD1; KS1/4; hEGP314; Major gastrointestinal tumor-associated protein GA733-2; M4S1; MK-1; Epithelial glycoprotein; KS 1/4 antigen; ESA; DIAR5; EGP314; Epithelial cell adhesion molecule; EGP-2; TROP1; CD antigen CD326 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediated in Citrate buffer (pH 6.0) * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Calculated MW | 35 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified by affinity chromatography. |
| Buffer | PBS and 0.02% Sodium azide |
| Preservative | 0.02% Sodium azide |
| Concentration | 1 mg/ml |
| Storage instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database links | |
|---|---|
| Gene Symbol | EPCAM |
| Gene Full Name | epithelial cell adhesion molecule |
| Background | EpCAM is a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008] |
| Function | EpCAM may act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E. [UniProt] |
| Highlight | Related Antibody Duos and Panels: ARG30305 Epithelial Marker Antibody Duo (Muc-1, EpCAM) ARG30311 Circulating Tumor Cells Biomarker Antibody Duo (EpCAM, HER2) Related products: EpCAM antibodies; EpCAM ELISA Kits; EpCAM Duos / Panels; Anti-Mouse IgG secondary antibodies; |
| Research Area | Controls and Markers antibody; Epithelial Marker antibody; Circulating Tumor Cells BioMarker antibody |
| PTM | Hyperglycosylated in carcinoma tissue as compared with autologous normal epithelia. Glycosylation at Asn-198 is crucial for protein stability. |
Images (3) Click the Picture to Zoom In
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ARG10517 anti-EpCAM antibody [AUA1] WB image
Western blot: 30 µg of SAS cell lysate stained with ARG10517 anti-EpCAM antibody [AUA1] at 1:100 dilution.
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ARG10517 anti-EpCAM antibody [AUA1] ICC/IF image
Immunofluorescence: SW1222 cells stained with ARG10517 anti-EpCAM antibody [AUA1].
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ARG10517 anti-EpCAM antibody [AUA1] IHC-P image
Immunohistochemistry: Paraffin-embedded HT29 Xenograft tumor stained with ARG10517 anti-EpCAM antibody [AUA1].
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Review for anti-EpCAM antibody [AUA1]
Application：WB
Sample：SAS cell lysate (RIPA + protease inhibiter)
Sample Loading Amount：30 μg
Primary Antibody Dilution Factor：1:100
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Conjugation of Secondary Antibody：HRP
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