ARG10517

anti-EpCAM antibody [AUA1]

anti-EpCAM antibody [AUA1] for ELISA,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Human

Controls and Markers antibody

Overview

Product Description

Mouse Monoclonal antibody [AUA1] recognizes EpCAM

Tested Reactivity Hu
Tested Application ELISA, FACS, ICC/IF, IHC-P
Specificity Cross reacts with breast carcinomas, kidney tubules, tonsil crypt epithelium and cells in some serious effusions. This is a good antibody for characterisation of all epithelial cells.
Host Mouse
Clonality Monoclonal
Clone AUA1
Isotype IgG1
Target Name EpCAM
Antigen Species Human
Immunogen LoVo cell line, a colon carcinoma cell line.
Conjugation Un-conjugated
Full Name epithelial cell adhesion molecule
Alternate Names MIC18; EGP; Tumor-associated calcium signal transducer 1; Epithelial glycoprotein 314; KSA; Ep-CAM; Epithelial cell surface antigen; Adenocarcinoma-associated antigen; HNPCC8; Cell surface glycoprotein Trop-1; EGP40; TACSTD1; KS1/4; hEGP314; Major gastrointestinal tumor-associated protein GA733-2; M4S1; MK-1; Epithelial glycoprotein; KS 1/4 antigen; ESA; DIAR5; EGP314; Epithelial cell adhesion molecule; EGP-2; TROP1; CD antigen CD326

Application Instructions

Application Suggestion
Tested Application
ELISAAssay-dependent.
FACS1µg for 10^6 cells. (methanol fixed cells)
ICC/IFAssay-dependent.
IHC-P5 µg/ml
Application Note IHC-P: Antigen Retrieval: Heat mediated in Citrate buffer (pH 6.0)
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 40 kDa

Properties

Form Liquid
Purification Purified by affinity chromatography.
Buffer PBS and 0.02% Sodium azide
Preservative 0.02% Sodium azide
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 4072 Human EPCAM

Swiss-port # P16422 Human Epithelial cell adhesion molecule

Gene Symbol EPCAM
Gene Full Name epithelial cell adhesion molecule
Background EPCAM is a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]
Function May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E. [Uniprot]
Highlight Related products:
EpCAM antibodies; EpCAM Duos / Panels; Anti-Mouse IgG secondary antibodies;
Research Area Controls and Markers antibody
PTM Hyperglycosylated in carcinoma tissue as compared with autologous normal epithelia. Glycosylation at Asn-198 is crucial for protein stability.

(2) Click the Picture to Zoom In

  • ARG10517 anti-EpCAM antibody [AUA1] ICC/IF image

    Immunofluorescence: SW1222 cells stained with ARG10517 anti-EpCAM antibody [AUA1].

  • ARG10517 anti-EpCAM antibody [AUA1] IHC-P image

    Immunohistochemistry: Paraffin-embedded HT29 Xenograft tumor stained with ARG10517 anti-EpCAM antibody [AUA1].

Clone References

Multiplex flow cytometry barcoding and antibody arrays identify surface antigen profiles of primary and metastatic colon cancer cell lines.

ICC/IF / Human

Sukhdeo K et al.
PLoS One.,  (2013)

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Matrix-insensitive protein assays push the limits of biosensors in medicine.

ELISA / 

Gaster RS et al.
Nat Med.,  (2009)

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Inhibition of androgen-independent prostate cancer cell growth is enhanced by combination therapy targeting Hedgehog and ErbB signalling.

ICC/IF / Human

Shaw G et al.
Cancer Cell Int.,  (2008)

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Expression of a cell surface antigen recognized by the monoclonal antibody AUA1 in bladder carcinoma: an immunohistochemical study.

Zorzos J et al.
Eur Urol.,  (1995)

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Detection of human cancer in an animal model using radio-labelled tumour-associated monoclonal antibodies.

Epenetos AA et al.
Br J Cancer.,  (1982)

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