anti-EpCAM antibody [SQab18103]

anti-EpCAM antibody [SQab18103] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Controls and Markers antibody; Epithelial Marker antibody; Circulating Tumor Cells BioMarker antibody


Product Description Recombinant Rabbit Monoclonal antibody [SQab18103] recognizes EpCAM
Tested Reactivity Hu
Tested Application IHC-P, WB
Host Rabbit
Clonality Monoclonal
Clone SQab18103
Isotype IgG
Target Name EpCAM
Antigen Species Human
Immunogen Synthetic peptide within aa. 1-100 of Human EpCAM.
Conjugation Un-conjugated
Alternate Names MIC18; EGP; Tumor-associated calcium signal transducer 1; Epithelial glycoprotein 314; KSA; Ep-CAM; Epithelial cell surface antigen; Adenocarcinoma-associated antigen; HNPCC8; Cell surface glycoprotein Trop-1; EGP40; TACSTD1; KS1/4; hEGP314; Major gastrointestinal tumor-associated protein GA733-2; M4S1; MK-1; Epithelial glycoprotein; KS 1/4 antigen; ESA; DIAR5; EGP314; Epithelial cell adhesion molecule; EGP-2; TROP1; CD antigen CD326

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:100 - 1:200
WB1:500 - 1:4000
Application Note IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0), primary antibody incubate at RT for 30 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 35 kDa


Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 4072 Human EPCAM

Swiss-port # P16422 Human Epithelial cell adhesion molecule

Gene Symbol EPCAM
Gene Full Name epithelial cell adhesion molecule
Background EpCAM is a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]
Function EpCAM may act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E. [UniProt]
Highlight Related products:
EpCAM antibodies; EpCAM ELISA Kits; EpCAM Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Related news:
Cancer Pathology Markers (SQ clones)
Research Area Controls and Markers antibody; Epithelial Marker antibody; Circulating Tumor Cells BioMarker antibody
Calculated MW 35 kDa
PTM Hyperglycosylated in carcinoma tissue as compared with autologous normal epithelia. Glycosylation at Asn-198 is crucial for protein stability. [UniProt]

Images (2) Click the Picture to Zoom In

  • ARG66353 anti-EpCAM antibody [SQab18103] WB image (Customer's Feedback)

    Western blot: HT-29 cell lysate stained with ARG66353 anti-EpCAM antibody [SQab18103] at 1:1000 dilution.

  • ARG66353 anti-EpCAM antibody [SQab18103] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded colon tissue stained with ARG66353 anti-EpCAM antibody [SQab18103]. Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0).

Customer's Feedback

nuts_pic      Excellent

Review for anti-EpCAM antibody [SQab18103]



Sample Loading Amount:20 μg

SDS Page Type:Denatured

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Conjugation of Secondary Antibody:HRP