anti-EpCAM antibody [SQab18103]
anti-EpCAM antibody [SQab18103] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Controls and Markers antibody; Epithelial Marker antibody; Circulating Tumor Cells BioMarker antibody
|Product Description||Recombinant Rabbit Monoclonal antibody [SQab18103] recognizes EpCAM|
|Tested Application||IHC-P, WB|
|Immunogen||Synthetic peptide within aa. 1-100 of Human EpCAM.|
|Alternate Names||MIC18; EGP; Tumor-associated calcium signal transducer 1; Epithelial glycoprotein 314; KSA; Ep-CAM; Epithelial cell surface antigen; Adenocarcinoma-associated antigen; HNPCC8; Cell surface glycoprotein Trop-1; EGP40; TACSTD1; KS1/4; hEGP314; Major gastrointestinal tumor-associated protein GA733-2; M4S1; MK-1; Epithelial glycoprotein; KS 1/4 antigen; ESA; DIAR5; EGP314; Epithelial cell adhesion molecule; EGP-2; TROP1; CD antigen CD326|
|Application Note||IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0), primary antibody incubate at RT for 30 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|Observed Size||35 kDa|
|Purification||Purification with Protein A.|
|Buffer||PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.|
|Preservative||0.01% Sodium azide|
|Stabilizer||40% Glycerol and 0.05% BSA|
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||epithelial cell adhesion molecule|
|Background||EpCAM is a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]|
|Function||EpCAM may act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E. [UniProt]|
EpCAM antibodies; EpCAM ELISA Kits; EpCAM Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Cancer Pathology Markers (SQ clones)
|Research Area||Controls and Markers antibody; Epithelial Marker antibody; Circulating Tumor Cells BioMarker antibody|
|Calculated MW||35 kDa|
|PTM||Hyperglycosylated in carcinoma tissue as compared with autologous normal epithelia. Glycosylation at Asn-198 is crucial for protein stability. [UniProt]|
Images (2) Click the Picture to Zoom In
ARG66353 anti-EpCAM antibody [SQab18103] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded colon tissue stained with ARG66353 anti-EpCAM antibody [SQab18103]. Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0).
ARG66353 anti-EpCAM antibody [SQab18103] WB image
Western blot: HT-29 cell lysate stained with ARG66353 anti-EpCAM antibody [SQab18103] at 1:1000 dilution.