ARG45736
anti-Epac1 antibody
anti-Epac1 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Monkey
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Epac1 |
|---|---|
| Tested Reactivity | Hu, Ms, Mk |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Epac1 |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human Epac1. |
| Conjugation | Un-conjugated |
| Alternate Names | RAPGEF3; Rap Guanine Nucleotide Exchange Factor 3; CAMP-GEFI; EPAC; Exchange Protein Directly Activated By CAMP 1; Bcm910; Rap1 Guanine-Nucleotide-Exchange Factor Directly Activated By CAMP; CAMP-Regulated Guanine Nucleotide Exchange Factor I; Rap Guanine Nucleotide Exchange Factor (GEF) 3; Exchange Factor Directly Activated By CAMP 1; EPAC 1; EPAC1; RAP Guanine-Nucleotide-Exchange Factor (GEF) 3; 9330170P05Rik; HSU79275; CGEF1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Observed Size | 104 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O95398 Human Rap guanine nucleotide exchange factor 3 Swiss-port # Q8VCC8 Mouse Rap guanine nucleotide exchange factor 3 |
|---|---|
| Gene Symbol | RAPGEF3 |
| Gene Full Name | Rap Guanine Nucleotide Exchange Factor 3 |
| Background | Enables guanyl-nucleotide exchange factor activity and protein domain specific binding activity. Involved in several processes, including Rap protein signal transduction; cellular response to cAMP; and regulation of syncytium formation by plasma membrane fusion. Located in several cellular components, including filopodium; lamellipodium; and microvillus. Implicated in nicotine dependence. Biomarker of congestive heart failure. [provided by Alliance of Genome Resources, Feb 2025] |
| Function | Guanine nucleotide exchange factor (GEF) for RAP1A and RAP2A small GTPases that is activated by binding cAMP. Through simultaneous binding of PDE3B to RAPGEF3 and PIK3R6 is assembled in a signaling complex in which it activates the PI3K gamma complex and which is involved in angiogenesis. Plays a role in the modulation of the cAMP-induced dynamic control of endothelial barrier function through a pathway that is independent on Rho-mediated signaling. Required for the actin rearrangement at cell-cell junctions, such as stress fibers and junctional actin. [UniProt] |
| Cellular Localization | Membrane. [UniProt] |
| Calculated MW | 104 kDa |
| PTM | Phosphoprotein. [UniProt] |
Images (4) Click the Picture to Zoom In
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ARG45736 anti-Epac1 antibody IHC-P image
Immunohistochemistry: Human lung cancer stained with ARG45736 anti-Epac1 antibody at 2 μg/ml dilution.
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ARG45736 anti-Epac1 antibody ICC/IF image
Immunofluorescence: T-47D stained with ARG45736 anti-Epac1 antibody at 5 μg/ml dilution.
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ARG45736 anti-Epac1 antibody WB image
Western blot: RAW264.7 stained with ARG45736 anti-Epac1 antibody at 0.5 μg/ml dilution.
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ARG45736 anti-Epac1 antibody FACS image
Flow Cytometry: MCF-7 stained with ARG45736 anti-Epac1 antibody at 1 µg/10^6 cells dilution.
