ARG51566

anti-Ezrin phospho (Tyr353) antibody

anti-Ezrin phospho (Tyr353) antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse

Cancer antibody; Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes Ezrin phospho (Tyr353)
Tested Reactivity Hu, Ms
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Ezrin
Antigen Species Human
Immunogen Peptide sequence around phosphorylation site of tyrosine 353 (Q-D-Y(p)-E-E) derived from Human EZRIN.
Conjugation Un-conjugated
Alternate Names Ezrin; p81; HEL-S-105; VIL2; CVL; Cytovillin; CVIL; Villin-2

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:100
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Buffer PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 22350 Mouse EZR

GeneID: 7430 Human EZR

Swiss-port # P15311 Human Ezrin

Swiss-port # P26040 Mouse Ezrin

Gene Symbol EZR
Gene Full Name ezrin
Background Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.
Function Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis. [UniProt]
Research Area Cancer antibody; Signaling Transduction antibody
Calculated MW 69 kDa
PTM Phosphorylated by tyrosine-protein kinases. Phosphorylation by ROCK2 suppresses the head-to-tail association of the N-terminal and C-terminal halves resulting in an opened conformation which is capable of actin and membrane-binding (By similarity).
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.

Images (2) Click the Picture to Zoom In

  • ARG51566 anti-Ezrin phospho (Tyr353) antibody WB image

    Western blot: Extracts from 293 cells untreated or treated with EGF stained with ARG51566 anti-Ezrin phospho (Tyr353) antibody.

  • ARG51566 anti-Ezrin phospho (Tyr353) antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51566 anti-Ezrin phospho (Tyr353) antibody (left) or the same antibody preincubated with blocking peptide (right).