ARG54382

anti-F1A alpha antibody

anti-F1A alpha antibody for Western blot,Immunohistochemistry and Human,Mouse,Rat

Cell Biology and Cellular Response antibody; Cell Death antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes F1A alpha
Tested Reactivity Hu, Ms, Rat
Tested Application IHC, WB
Specificity This antibody recognizes human, mouse, and rat F1A (70kDa).
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name F1A alpha
Antigen Species Human
Immunogen Peptide corresponding to aa 609-622 of human F1A (accession no. AAF05314). This sequence is identical to that of mouse.
Conjugation Un-conjugated
Alternate Names Fem-1-like death receptor-binding protein alpha; F1A-alpha; Protein fem-1 homolog B; F1A-ALPHA; Fem-1-like in apoptotic pathway protein alpha; FEM1-beta; FEM1b; F1AA

Application Instructions

Application Suggestion
Tested Application Dilution
IHCAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse and Rat liver

Properties

Form Liquid
Purification Immunoaffinity chroma-tography
Buffer PBS (pH 7.4) and 0.02% Sodium azide
Preservative 0.02% Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 10116 Human FEM1B

GeneID: 14155 Mouse FEM1B

GeneID: 315745 Rat FEM1B

Gene Symbol FEM1B
Gene Full Name fem-1 homolog b (C. elegans)
Background Fas and tumor necrosis factor receptor 1 (TNFR1) are two members of the family of death receptors. A novel protein that associates with the intracellular domains of Fas and TNFR1 has been designated F1A (or FEM1 ). F1A is cleaved by CED-3 and caspase, and it associates with CED-4 and its mammalian homologue Apaf-1. Overexpression of F1A induces apoptosis. F1A is expressed in a variety of human and mouse tissues.
Function Component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit. Involved in apoptosis by acting as a death receptor-associated protein that mediates apoptosis. Also involved in glucose homeostasis in pancreatic islet. Functions as an adapter/mediator in replication stress-induced signaling that leads to the activation of CHEK1. [UniProt]
Research Area Cell Biology and Cellular Response antibody; Cell Death antibody
Calculated MW 70 kDa

Images (2) Click the Picture to Zoom In

  • ARG54382 anti-F1A alpha antibody WB image

    Western Blot: A:mouse liver ; B: rat liver stained with ARG54382 anti-F1A alpha antibody at 1 μg/ml dilution.

  • ARG54382 anti-F1A alpha antibody IHC image

    Immunohistochemistry: mouse liver stained with ARG54382 anti-F1A alpha antibody at 5 μg/ml dilution.