ARG53014

anti-FBXW7 antibody [SP237]

anti-FBXW7 antibody [SP237] for IHC-Formalin-fixed paraffin-embedded sections,Flow cytometry and Human

Cancer antibody; Cell Biology and Cellular Response antibody

Overview

Product Description Rabbit Monoclonal antibody [SP237] recognizes FBXW7
Tested Reactivity Hu
Tested Application FACS, IHC-P
Host Rabbit
Clonality Monoclonal
Clone SP237
Isotype IgG
Target Name FBXW7
Antigen Species Human
Immunogen Synthetic peptide derived from the internal region of human FBXW7 protein.
Conjugation Un-conjugated
Alternate Names AGO; F-box protein FBX30; hCdc4; FBW7; FBW6; F-box/WD repeat-containing protein 7; SEL-10; hAgo; FBX30; SEL10; FBXO30; F-box and WD-40 domain-containing protein 7; CDC4; FBXW6; Archipelago homolog

Application Instructions

Application Suggestion
Tested Application Dilution
FACSAssay-Dependent
IHC-P1:100
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Endometrial Adenocarcinoma, Lung Squamous Cell Carcinoma

Properties

Form Liquid
Purification Purified by protein A/G
Buffer PBS (pH 7.6), 1% BSA and < 0.1% Sodium azide
Preservative < 0.1% Sodium azide
Stabilizer 1% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 55294 Human FBXW7

Swiss-port # Q969H0 Human F-box/WD repeat-containing protein 7

Background F-box/WD repeat-containing protein 7 (Fbxw7) is part of an ubiquitin-protein ligase complex. The complex ubiquitinates and degrades several important oncogenes such as mTOR, MYC, AURKA, JUN, and NOTCH1. Fbxw7 cooperates with PTEN for tumor suppression and is widely expressed in many normal tissues. The loss of fbxw7 is associated with malignant progression in a variety of tumors, including carcinoma of colorectum, lung, stomach, bile duct, endometrium, pancreas, breast and glioblastoma.
Cellular Localization Cytoplasm, Nucleus, Nucleolus
Research Area Cancer antibody; Cell Biology and Cellular Response antibody
Calculated MW 80 kDa
PTM Phosphorylation at Thr-205 promotes interaction with PIN1, leading to disrupt FBXW7 dimerization and promoting FBXW7 autoubiquitination and degradation (PubMed:22608923).
Ubiquitinated: autoubiquitinates following phosphorylation at Thr-205 and subsequent interaction with PIN1. Ubiquitination leads to its degradation (PubMed:22608923).

Images (1) Click the Picture to Zoom In

  • ARG53014 anti-FBXW7 antibody [SP237] IHC-P image

    Immunohistochemistry: Human Endometrial Adenocarcinoma stained with FBXW7 antibody [SP237] (ARG53014)