ARG54167

anti-FEN1 antibody

anti-FEN1 antibody for Western blot,ICC/IF and Human,Mouse,Rat,Monkey,Hamster

Gene Regulation antibody

Overview

Product Description Mouse Monoclonal antibody recognizes FEN1
Tested Reactivity Hu, Ms, Rat, Hm, Mk
Tested Application ICC/IF, WB
Host Mouse
Clonality Monoclonal
Isotype IgG1
Target Name FEN1
Antigen Species Human
Immunogen Purified recombinant human FEN-1 protein fragments expressed in E.coli.
Conjugation Un-conjugated
Alternate Names hFEN-1; Maturation factor 1; Flap endonuclease 1; DNase IV; EC 3.1.-.-; FEN-1; MF1; Flap structure-specific endonuclease 1; RAD2

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:400
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 45 kDa

Properties

Form Liquid
Purification Affinity purified
Buffer PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Concentration 1.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2237 Human FEN1

GeneID: 84490 Rat FEN1

Swiss-port # P39748 Human Flap endonuclease 1

Swiss-port # Q5XIP6 Rat Flap endonuclease 1

Gene Symbol FEN1
Gene Full Name flap structure-specific endonuclease 1
Background Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair.During DNA replication,cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment.It enters the flap from the 5'-end and then tracks to cleave the flap base,leaving a nick for ligation.Also involved in the long patch base excision repair (LP-BER) pathway,by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap.Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions.Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA,and exhibits RNase H activity.Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.
Function Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA. [UniProt]
Cellular Localization Isoform 1: Nucleus > nucleolus. Nucleus > nucleoplasm. Note: Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage. Isoform FENMIT: Mitochondrion.
Research Area Gene Regulation antibody
Calculated MW 43 kDa
PTM Acetylated by EP300. Acetylation inhibits both endonuclease and exonuclease activity. Acetylation also reduces DNA-binding activity but does not affect interaction with PCNA or EP300.
Phosphorylation upon DNA damage induces relocalization to the nuclear plasma. Phosphorylation at Ser-187 by CDK2 occurs during late S-phase and results in dissociation from PCNA.
Methylation at Arg-192 by PRMT5 impedes Ser-187 phosphorylation and increases interaction with PCNA.

Images (2) Click the Picture to Zoom In

  • ARG54167 anti-FEN1 antibody ICC/IF image

    Immunofluorescence: HeLa cells fixed with 4% Paraformaldehyde and stained with ARG54167 anti-FEN1 antibody at 1:400 dilution.

  • ARG54167 anti-FEN1 antibody WB image

    Western blot: HeLa cell lysate stained with ARG54167 anti-FEN1 antibody at 1:1000 dilution.