anti-FGFR3 antibody

anti-FGFR3 antibody for IHC-Formalin-fixed paraffin-embedded sections and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Signaling Transduction antibody


Product Description Rabbit Polyclonal antibody recognizes FGFR3
Tested Reactivity Hu
Tested Application IHC-P
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name FGFR3
Antigen Species Human
Immunogen Synthetic peptide derived from internal region of human FGFR-3 protein.
Conjugation Un-conjugated
Alternate Names CEK2; CD antigen CD333; FGFR-3; ACH; JTK4; Fibroblast growth factor receptor 3; CD333; EC; HSFGFR3EX

Application Instructions

Application Suggestion
Tested Application Dilution
Application Note IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Incubation Time: 30 min at RT.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Breast, Breast Carcinoma


Form Liquid
Purification Immunogen affinity purified
Buffer PBS (pH 7.6), 1% BSA and < 0.1% Sodium azide
Preservative < 0.1% Sodium azide
Stabilizer 1% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 2261 Human FGFR3

Swiss-port # P22607 Human Fibroblast growth factor receptor 3

Background Fibroblast growth factors (FGFs) are members of a large family of structurally related polypeptides that are potent physiological regulators of growth and differentiation for a wide variet of cells of mesodermal, ectodermal and endodermal origin. Four genes encoding for high affinity cell surface FGF receptors (FGFRs) have been identified: FGFR-1, FGFR-2, FGFR-3 and FGFR-4.FGFRs are emembers of the tyrosine kinase family of growth factor receptors. FGFR-3 is widely expressed in many fetal and adult human and animal tissues.
Cellular Localization Cytoplasm
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Signaling Transduction antibody
Calculated MW 88 kDa
PTM Autophosphorylated. Binding of FGF family members together with heparan sulfate proteoglycan or heparin promotes receptor dimerization and autophosphorylation on tyrosine residues. Autophosphorylation occurs in trans between the two FGFR molecules present in the dimer. Phosphorylation at Tyr-724 is essential for stimulation of cell proliferation and activation of PIK3R1, STAT1 and MAP kinase signaling. Phosphorylation at Tyr-760 is required for interaction with PIK3R1 and PLCG1.
Ubiquitinated. Is rapidly ubiquitinated after ligand binding and autophosphorylation, leading to receptor internalization and degradation. Subject to both proteasomal and lysosomal degradation.
N-glycosylated in the endoplasmic reticulum. The N-glycan chains undergo further maturation to an Endo H-resistant form in the Golgi apparatus.

Images (1) Click the Picture to Zoom In

  • ARG53020 anti-FGFR3 antibody IHC-P image

    Immunohistochemistry: Human Breast stained with ARG53020 anti-FGFR3 antibody.