ARG22264

anti-FKBP4 / FKBP52 antibody [Hi52C]

anti-FKBP4 / FKBP52 antibody [Hi52C] for Western blot,Immunohistochemistry,ICC/IF,Immunoprecipitation and Dog,Human,Mouse,Rat,Hamster

Overview

Product Description Mouse Monoclonal antibody [Hi52C] recognizes FKBP4 / FKBP52
Tested Reactivity Hu, Ms, Rat, Dog, Hm
Tested Application ICC/IF, IHC, IP, WB
Specificity Detects ~52kDa. Heavy chain migrates close to FKBP52 on SDS PAGE.
Host Mouse
Clonality Monoclonal
Clone Hi52C
Isotype IgG
Target Name FKBP4 / FKBP52
Antigen Species Human
Immunogen Synthetic peptide from Human FKBP52
Conjugation Un-conjugated
Alternate Names Hsp56; FKBP59; HBI; FKBP-52; 52 kDa FK506-binding protein; PPIase FKBP4; FKBP52; Rotamase; FKBP51; Immunophilin FKBP52; 51 kDa FK506-binding protein; EC 5.2.1.8; PPIase; p59; FKBP-4; 52 kDa FKBP; 59 kDa immunophilin; Peptidyl-prolyl cis-trans isomerase FKBP4; FK506-binding protein 4; HSP-binding immunophilin; p52

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:1000
IHC1:250
IP5 µg/ml
WB1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS, 0.09% Sodium azide and 50% Glycerol
Preservative 0.09% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 14228 Mouse FKBP4

GeneID: 2288 Human FKBP4

GeneID: 260321 Rat FKBP4

Gene Symbol FKBP4
Gene Full Name FK506 binding protein 4, 59kDa
Background The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This encoded protein is a cis-trans prolyl isomerase that binds to the immunosuppressants FK506 and rapamycin. It has high structural and functional similarity to FK506-binding protein 1A (FKBP1A), but unlike FKBP1A, this protein does not have immunosuppressant activity when complexed with FK506. It interacts with interferon regulatory factor-4 and plays an important role in immunoregulatory gene expression in B and T lymphocytes. This encoded protein is known to associate with phytanoyl-CoA alpha-hydroxylase. It can also associate with two heat shock proteins (hsp90 and hsp70) and thus may play a role in the intracellular trafficking of hetero-oligomeric forms of the steroid hormone receptors. This protein correlates strongly with adeno-associated virus type 2 vectors (AAV) resulting in a significant increase in AAV-mediated transgene expression in human cell lines. Thus this encoded protein is thought to have important implications for the optimal use of AAV vectors in human gene therapy. The human genome contains several non-transcribed pseudogenes similar to this gene. [provided by RefSeq, Sep 2008]
Function Immunophilin protein with PPIase and co-chaperone activities. Component of steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). May play a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors between cytoplasm and nuclear compartments. The isomerase activity controls neuronal growth cones via regulation of TRPC1 channel opening. Acts also as a regulator of microtubule dynamics by inhibiting MAPT/TAU ability to promote microtubule assembly. May have a protective role against oxidative stress in mitochondria. [UniProt]
Cellular Localization Cytoplasm, Nucleus
Calculated MW 52 kDa
PTM Phosphorylation by CK2 results in loss of HSP90 binding activity.

Images (2) Click the Picture to Zoom In

  • ARG22264 anti-FKBP4 / FKBP52 antibody [Hi52C] WB image

    Western blot: 15 µg of Rat brain, heart, kidney, liver, pancreas, skeletal muscle, spleen, testes, and thymus, H9C2, NIH 3T3, and Pam212 lysates stained with ARG22264 anti-FKBP4 / FKBP52 antibody [Hi52C] at 1.5 µg/ml dilution (2 hours at RT).

  • ARG22264 anti-FKBP4 / FKBP52 antibody [Hi52C] WB image

    Western blot: 15 µg of Human cell lysates stained with ARG22264 anti-FKBP4 / FKBP52 antibody [Hi52C] at 1.5 µg/ml dilution (2 hours at RT).