anti-FMRP phospho (Ser499) antibody
anti-FMRP phospho (Ser499) antibody for Western blot and Rat
Gene Regulation antibody; Neuroscience antibody
|Product Description||Rabbit Polyclonal antibody recognizes FMRP phospho (Ser499)|
|Predict Reactivity||Hu, Ms, Bov, Chk, Dog, NHuPrm, Sheep, Xenopus laevis, Zfsh|
|Immunogen||Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser499 conjugated to KLH|
|Alternate Names||POF1; Fragile X mental retardation protein 1; FRAXA; Protein FMR-1; POF; FMRP|
|Application Note||Specific for ~71k FMRP protein phosphorylated at Ser499. Immunolabeling of the FMRP protein is completely eliminated by lambda-phosphatase.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|Buffer||10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol|
|Stabilizer||0.1 mg/ml BSA, 50% Glycerol|
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||fragile X mental retardation 1|
|Background||Fragile X Mental Retardation Protein (FMRP) is an RNA-binding protein that plays an essential role in cognitive brain function. Mutations in the FMR1 gene, which codes for FMRP, can result in fragile X syndrome, autism, as well as other cognitive deficits (Brown et al.,1998, Goodlin-Jones et al., 2004). Phosphorylation of the highly conserved Ser499 has been shown to trigger hierarchical phosphorylation of nearby serines and may play a role in suppressing target mRNA translation (Ceman et al., 2003, Narayanan et al. 2008).|
|Research Area||Gene Regulation antibody; Neuroscience antibody|
|Calculated MW||71 kDa|
|PTM||Phosphorylated (PubMed:14532325). Phosphorylated on several serine residues. Phosphorylation at Ser-500 is required for phosphorylation of other nearby serine residues. Phosphorylation has no effect on the binding of individual mRNA species, but may affect the association with polyribosome. Unphosphorylated FMR1 is associated with actively translating polyribosome, whereas a fraction of phosphorylated FMR1 is associated with apparently stalled polyribosome. Dephosphorylation by an activated phosphatase may release the FMR1-mediated translational repression and allow synthesis of a locally required protein at snypases (By similarity).
Monoubiquitinated. Polyubiquitinated. Ubiquitinated and targeted for proteasomal degradation after activation of metabotropic glutamate receptor (mGluR).
Methylated; methylation is necessary for heterodimerization with FXR1, association with polyribosomes, recruitment into stress granules and translation of FMR1 target mRNAs (PubMed:16636078). Methylated by PRMT1, PRMT3 and PRMT4, in vitro (PubMed:16922515).
Isoform 10: Undergoes proteolytic cleavage; may be specifically cleaved by calpain-1/CAPN1 in cajal bodies (PubMed:24204304).
Images (1) Click the Picture to Zoom In
ARG52281 anti-FMRP phospho (Ser499) antibody WB image
Western blot: Rat hippocampal lysate showing specific immunolabeling of the ~71k FMRP protein phosphorylated at Ser 499 (Control) stained with ARG52281 anti-FMRP phospho (Ser499) antibody.
The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: λ-Ptase).
The blot is identical to the control except that the lysate was incubated in λ-Ptase (400 units/100ul lysate for 30 min) before being exposed to the FMRP Ser 499 antibody.
The immunolabeling is completely eliminated by treatment with λ-Ptase.