ARG10712

anti-FOX3 / NeuN antibody

anti-FOX3 / NeuN antibody for ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse,Rat,Cow,Horse,Pig

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anti-FOX3 / NeuN antibody

Rabbit Polyclonal antibody recognizes FOX3 / NeuN

ARG10712 Arigo biolaboratories
4.0 out of 5 based on 1
anti-FOX3 / NeuN antibody is rated 4.0 out of 5 by 1.

anti-FOX3 / NeuN antibody

Overview

Product Description

Rabbit Polyclonal antibody recognizes FOX3 / NeuN

Tested Reactivity Hu, Ms, Rat, Cow, Hrs, Pig
Predict Reactivity Chk
Tested Application ICC/IF, IHC-Fr, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name FOX3 / NeuN
Antigen Species Human
Immunogen N-terminal 100 aa. of Human Fox3 expressed in and purified from E. coli.
Conjugation Un-conjugated
Full Name RNA binding protein, fox-1 homolog (C. elegans) 3
Alternate Names RNA binding protein fox-1 homolog 3; NEUN; FOX-3; HRNBP3; Fox-1 homolog C; FOX3

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:500 - 1:1000
IHC-Fr1:500 - 1:1000
WB1:5000 - 1:10000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification.
Buffer PBS and 50% Glycerol.
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 146713 Human RBFOX3

GeneID: 52897 Mouse RBFOX3

Swiss-port # A6NFN3 Human RNA binding protein fox-1 homolog 3

Swiss-port # Q8BIF2 Mouse RNA binding protein fox-1 homolog 3

Gene Symbol RBFOX3
Gene Full Name RNA binding protein, fox-1 homolog (C. elegans) 3
Function RNA-binding protein that regulates alternative splicing events. [UniProt]

Images (3) Click the Picture to Zoom In

  • ARG10712 anti-FOX3 / NeuN antibody WB image

    Western blot: 30 µg of U87, Mouse brain and Rat brain lysates stained with ARG10712 anti-FOX3 / NeuN antibody at 1:5000 dilution.

  • ARG10712 anti-FOX3 / NeuN antibody IHC-Fr image

    Immunohistochemistry: Frozen section of Adult Mouse cerebellum stained with ARG10712 anti-FOX3 / NeuN antibody (red) at 1:5000 dilution and costained with ARG52313 anti-GFAP antibody (green) at 1:5000 dilution. (Sample preparation: Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours and 45 µm free-floating sections were stained with above antibodies.)

    The FOX3 / NeuN antibody stains the nuclei of neurons in the cerebellar granule layer. The GFAP antibody stains the processes of Bergmann glia in the molecular layer and astroglia in the granule and white matter layers.

  • ARG10712 anti-FOX3 / NeuN antibody IHC-Fr image

    Immunohistochemistry: Paraformaldehyde-fixed frozen section of adult Rat brain stem stained with ARG10712 anti-FOX3 / NeuN antibody (red) and co-stained for MAP2 (green). DNA is shown in blue. Note that RPCA-Fox3 stains neuronal nuclei and distal perikarya and that the MAP2 antibody stains the dendrites extending from these cells.

Customer's Feedback

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Review for anti-FOX3 / NeuN antibody

Application:WB

Sample:U87, Mouse brain and Rat brain

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:5000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC