anti-FOXC2 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human
Developmental Biology antibody; Gene Regulation antibody; Metabolism antibody
|Product Description||Goat Polyclonal antibody recognizes FOXC2|
|Predict Reactivity||Ms, Rat, Bov, Dog|
|Tested Application||IHC-P, WB|
|Alternate Names||LD; FKHL14; Transcription factor FKH-14; Forkhead box protein C2; MFH-1; MFH1; MFH-1 protein; Mesenchyme fork head protein 1; Forkhead-related protein FKHL14|
|Application Note||WB: Recommend incubate at RT for 1h. In transfected HEK293 transiently expressing full-length Human FOXC2 (myc and DYKDDDDK tagged), a band of approx. 65 kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration, 0.1-0.2 µg/ml
IHC-P: In paraffin embedded Human Bresast cancer shows nuclear staning wirth some weak cytoplasm staining. Recommended concentration, 2-4 µg/ml.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|Purification||Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.|
|Buffer||Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA|
|Preservative||0.02% Sodium azide|
|Storage instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Background||This gene belongs to the forkhead family of transcription factors which is characterized by a distinct DNA-binding forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in the development of mesenchymal tissues. [provided by RefSeq, Jul 2008]|
|Research Area||Developmental Biology antibody; Gene Regulation antibody; Metabolism antibody|
|PTM||Phosphorylation regulates FOXC2 transcriptional activity by promoting its recruitment to chromatin.|
Images (2) Click the Picture to Zoom In
ARG63332 anti-FOXC2 antibody WB image
Western Blot: HEK293 lysate (10ug protein in RIPA buffer) over expressing Human FOXC2 with DYKDDDDK tag stained with ARG63332 anti-FOXC2 antibody (0.1ug/ml) in Lane A overexpressing HEK293 cell lysate standed with anti- DYKDDDDK Tag (1/1000) in lane C. Mock-transfected HEK293 stained with ARG63332 anti-FOXC2 antibody (1mg/ml) in Lane B. Primary incubations were for 1 hour.
ARG63332 anti-FOXC2 antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Breast cancer. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63332 anti-FOXC2 antibody at 2 µg/ml dilution followed by HRP-staining.