anti-FOXC2 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Developmental Biology antibody; Gene Regulation antibody; Metabolism antibody; Chondrogenesis Study antibody
|Product Description||Goat Polyclonal antibody recognizes FOXC2|
|Predict Reactivity||Cow, Rat, Dog|
|Tested Application||FACS, IHC-P, WB|
|Alternate Names||LD; FKHL14; Transcription factor FKH-14; Forkhead box protein C2; MFH-1; MFH1; MFH-1 protein; Mesenchyme fork head protein 1; Forkhead-related protein FKHL14|
|Application Note||WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|Purification||Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.|
|Buffer||Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA|
|Preservative||0.02% Sodium azide|
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Background||This gene belongs to the forkhead family of transcription factors which is characterized by a distinct DNA-binding forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in the development of mesenchymal tissues. [provided by RefSeq, Jul 2008]|
|Highlight||Related Antibody Duos and Panels:
ARG30314 Chondrogenesis Marker Antibody Panel
FOXC2 antibodies; FOXC2 Duos / Panels; Anti-Goat IgG secondary antibodies;
|Research Area||Developmental Biology antibody; Gene Regulation antibody; Metabolism antibody; Chondrogenesis Study antibody|
|Calculated MW||54 kDa|
|PTM||Phosphorylation regulates FOXC2 transcriptional activity by promoting its recruitment to chromatin.|
Images (2) Click the Picture to Zoom In
ARG63332 anti-FOXC2 antibody WB image
Western blot: 35 µg of HEK293 nucleus (A) and Human pancreas (B, negative control) lysates (in RIPA buffer) stained with ARG63332 anti-FOXC2 antibody at 2 µg/ml (A) and 1 µg/ml (B) dilutions and incubated at RT for 1 hour.
ARG63332 anti-FOXC2 antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed HEK293 cells permeabilized with 0.5% Triton. Cells were stained with ARG63332 anti-FOXC2 antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).