ARG63332

anti-FOXC2 antibody

anti-FOXC2 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Developmental Biology antibody; Gene Regulation antibody; Metabolism antibody; Chondrogenesis Study antibody
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Overview

Product Description Goat Polyclonal antibody recognizes FOXC2
Tested Reactivity Hu
Predict Reactivity Cow, Rat, Dog
Tested Application FACS, IHC-P, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name FOXC2
Antigen Species Human
Immunogen RHAAPYSYDCTKY
Conjugation Un-conjugated
Alternate Names LD; FKHL14; Transcription factor FKH-14; Forkhead box protein C2; MFH-1; MFH1; MFH-1 protein; Mesenchyme fork head protein 1; Forkhead-related protein FKHL14

Application Instructions

Application Suggestion
Tested Application Dilution
FACS10 µg/ml
IHC-PAssay-dependent
WB1 - 2 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 54 kDa

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 2303 Human FOXC2

Swiss-port # Q99958 Human Forkhead box protein C2

Background This gene belongs to the forkhead family of transcription factors which is characterized by a distinct DNA-binding forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in the development of mesenchymal tissues. [provided by RefSeq, Jul 2008]
Highlight Related Antibody Duos and Panels:
ARG30314 Chondrogenesis Marker Antibody Panel
Related products:
FOXC2 antibodies; FOXC2 Duos / Panels; Anti-Goat IgG secondary antibodies;
Research Area Developmental Biology antibody; Gene Regulation antibody; Metabolism antibody; Chondrogenesis Study antibody
PTM Phosphorylation regulates FOXC2 transcriptional activity by promoting its recruitment to chromatin.

Images (2) Click the Picture to Zoom In

  • ARG63332 anti-FOXC2 antibody WB image

    Western blot: 35 µg of HEK293 nucleus (A) and Human pancreas (B, negative control) lysates (in RIPA buffer) stained with ARG63332 anti-FOXC2 antibody at 2 µg/ml (A) and 1 µg/ml (B) dilutions and incubated at RT for 1 hour.

  • ARG63332 anti-FOXC2 antibody FACS image

    Flow Cytometry: Paraformaldehyde-fixed HEK293 cells permeabilized with 0.5% Triton. Cells were stained with ARG63332 anti-FOXC2 antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).

Specific References

Expression and clinical significance of FOXC2 and E-cad in colon cancer tissues (FOXC2和E-cad在结肠癌组织中的表达及临床意义)

IHC-P / Human

Wang Jing-Miao (王静苗) et al.
Chinese Clinical Oncology (临床肿瘤学杂志), 21(8),  (2016)

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