ARG63332

anti-FOXC2 antibody

anti-FOXC2 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human

Developmental Biology antibody; Gene Regulation antibody; Metabolism antibody
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Overview

Product Description Goat Polyclonal antibody recognizes FOXC2
Tested Reactivity Hu
Predict Reactivity Ms, Rat, Bov, Dog
Tested Application IHC-P, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name FOXC2
Antigen Species Human
Immunogen RHAAPYSYDCTKY
Conjugation Un-conjugated
Alternate Names LD; FKHL14; Transcription factor FKH-14; Forkhead box protein C2; MFH-1; MFH1; MFH-1 protein; Mesenchyme fork head protein 1; Forkhead-related protein FKHL14

Application Instructions

Application Note WB: Recommend incubate at RT for 1h. In transfected HEK293 transiently expressing full-length Human FOXC2 (myc and DYKDDDDK tagged), a band of approx. 65 kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration, 0.1-0.2 µg/ml
IHC-P: In paraffin embedded Human Bresast cancer shows nuclear staning wirth some weak cytoplasm staining. Recommended concentration, 2-4 µg/ml.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 2303 Human FOXC2

Swiss-port # Q99958 Human Forkhead box protein C2

Background This gene belongs to the forkhead family of transcription factors which is characterized by a distinct DNA-binding forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in the development of mesenchymal tissues. [provided by RefSeq, Jul 2008]
Research Area Developmental Biology antibody; Gene Regulation antibody; Metabolism antibody
PTM Phosphorylation regulates FOXC2 transcriptional activity by promoting its recruitment to chromatin.

Images (2) Click the Picture to Zoom In

  • ARG63332 anti-FOXC2 antibody WB image

    Western blot: 10 µg of HEK293 cell lysate (in RIPA buffer) overexpressing Human FOXC2 with DYKDDDDK tag stained with ARG63332 anti-FOXC2 antibody at 0.1 µg/ml dilution (Lane A); Mock-transfected HEK293 stained with primary antibodies (Lane B); Overexpressing HEK293 cell lysate stained with anti-DYKDDDDK Tag at 1:1000 dilution (Lane C). Primary antibodies were incubated at RT for 1 hour.

  • ARG63332 anti-FOXC2 antibody IHC-P image

    Immunohistochemistry: paraffin embedded Human Breast cancer. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63332 anti-FOXC2 antibody at 2 µg/ml dilution followed by HRP-staining.

Specific References

Expression and clinical significance of FOXC2 and E-cad in colon cancer tissues (FOXC2和E-cad在结肠癌组织中的表达及临床意义)

IHC-P / Human

Wang Jing-Miao (王静苗) et al.
Chinese Clinical Oncology (临床肿瘤学杂志), 21(8),  (2016)

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