anti-Factor V Heavy chain antibody [B10]

anti-Factor V Heavy chain antibody [B10] for ELISA and Human

Cell Biology and Cellular Response antibody


Product Description Mouse Monoclonal antibody [B10] recognizes Factor V Heavy chain
Tested Reactivity Hu
Tested Application ELISA
Specificity This antibody reacts with the activation C peptide (mw ~150,000) in the heavy chain of thrombin-cleaved human Factor V. KD = 1.15 x 10-10 mol/L.
Host Mouse
Clonality Monoclonal
Clone B10
Isotype IgG1
Target Name Factor V Heavy chain
Antigen Species Human
Immunogen Purified human Factor V.
Conjugation Un-conjugated
Alternate Names FVL; Activated protein C cofactor; PCCF; Coagulation factor V; THPH2; Proaccelerin, labile factor; RPRGL1

Application Instructions

Application Note This antibody may be used in ELISA, immunohistochemistry, and autoradiography. Other applications are under investigation.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Protein G-purified
Buffer PBS (pH 7.4)
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 2153 Human F5

Swiss-port # P12259 Human Coagulation factor V

Gene Symbol F5
Gene Full Name coagulation factor V (proaccelerin, labile factor)
Background This gene encodes an essential cofactor of the blood coagulation cascade. This factor circulates in plasma, and is converted to the active form by the release of the activation peptide by thrombin during coagulation. This generates a heavy chain and a light chain which are held together by calcium ions. The activated protein is a cofactor that participates with activated coagulation factor X to activate prothrombin to thrombin. Defects in this gene result in either an autosomal recessive hemorrhagic diathesis or an autosomal dominant form of thrombophilia, which is known as activated protein C resistance. [provided by RefSeq, Oct 2008]
Function Central regulator of hemostasis. It serves as a critical cofactor for the prothrombinase activity of factor Xa that results in the activation of prothrombin to thrombin. [UniProt]
Research Area Cell Biology and Cellular Response antibody
Calculated MW 252 kDa
PTM Thrombin activates factor V proteolytically to the active cofactor, factor Va (formation of a heavy chain at the N-terminus and a light chain at the C-terminus).
Sulfation is required for efficient thrombin cleavage and activation and for full procoagulant activity.
Activated protein C inactivates factor V and factor Va by proteolytic degradation.
Phosphorylated by FAM20C in the extracellular medium.