ARG10090

anti-Factor X antibody [F10-1]

anti-Factor X antibody [F10-1] for ELISA,Western blot and Human

Cell Biology and Cellular Response antibody

Overview

Product Description Mouse Monoclonal antibody [F10-1] recognizes Factor X
Tested Reactivity Hu
Tested Application ELISA, WB
Host Mouse
Clonality Monoclonal
Clone F10-1
Isotype IgG1,k
Target Name Factor X
Antigen Species Human
Immunogen Human Factor X
Conjugation Un-conjugated
Full Name coagulation factor X
Alternate Names FX; Stuart factor; EC 3.4.21.6; Stuart-Prower factor; FXA; Coagulation factor X

Application Instructions

Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Protein G affinity purified
Buffer 0.01M PBS (pH 7.2)
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 2159 Human F10

Swiss-port # P00742 Human Coagulation factor X

Gene Symbol F10
Gene Full Name coagulation factor X
Background Factor X is the first enzyme in the common pathway of blood coagulation. It can be activated by Factor IXa (with Factor VIII cofactor) of the contact pathway and by Factor VIIa of the tissue factor pathway. After activation, It cleaves prothrombin to form thrombin. Thrombin not only transforms Fibrinogen to form Fibrin and but also activates Factor XIII to stabilize Fibrin in the thrombus.
Function Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting. [UniProt]
Research Area Cell Biology and Cellular Response antibody
PTM The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.
The activation peptide is cleaved by factor IXa (in the intrinsic pathway), or by factor VIIa (in the extrinsic pathway).
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.

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  • ARG10090 anti-Factor X antibody [F10-1] WB image

    Western Blot: Purified human Factor-X antigen (200 ng/well) stained with anti-Factor X antibody [F10-1] (ARG10090) at 1 μg/mL

  • ARG10090 anti-Factor X antibody [F10-1] WB image

    Western Blot: 1,2: Anti-human Factor-X ab used at 0.2 μg/mL dilution 3,4: Anti-human Factor-X ab used at 0.5 μg/mL dilution 5,6: Anti-human Factor-X ab used at 1 μg/mL dilution stained with anti-Factor X antibody [F10-1] (ARG10090)