anti-Fatso antibody [5-2H10]

anti-Fatso antibody [5-2H10] for ICC/IF,Western blot and Human,Mouse,Rat

Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody


Product Description Mouse Monoclonal antibody [5-2H10] recognizes Fatso
Tested Reactivity Hu, Ms, Rat
Predict Reactivity Bov, Dog, Hrs, NHuPrm, Sheep
Tested Application ICC/IF, WB
Host Mouse
Clonality Monoclonal
Clone 5-2H10
Isotype IgG
Target Name Fatso
Antigen Species Human
Immunogen Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH
Conjugation Un-conjugated
Alternate Names EC 1.14.11.-; Alpha-ketoglutarate-dependent dioxygenase FTO; Fat mass and obesity-associated protein; ALKBH9

Application Instructions

Application Suggestion
Tested Application Dilution
Application Note Specific for the ~58 kDa Fto protein in Western blots of Rat testes lysate. The antibody has also been used for immunocytochemistry with neuronal progenitor cells.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Protein G purified
Buffer 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol
Stabilizer 0.1 mg/ml BSA, 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 26383 Mouse FTO

GeneID: 291905 Rat FTO

GeneID: 79068 Human FTO

Gene Symbol FTO
Gene Full Name fat mass and obesity associated
Background The FTO gene is the most robust gene for common obesity characterized to date. FTO gene expression has been found to be significantly upregulated in the hypothalamus of rats after food deprivation and strongly negatively correlated with the expression of orexin peptide which is involved in the stimulation of food intake (Fredricksson R et al., 2008). Deletion analysis of FTO gene in mice showed that Fto is functionally involved in the control of both energy intake and energy expenditure (Fischer J et al., 2009)
Research Area Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody
Calculated MW 58 kDa

Images (1) Click the Picture to Zoom In

  • ARG52285 anti-Fatso antibody [5-2H10] WB image

    Western blot: Rat testis lysate showing specific immunolabeling of the ~ 58 kDa Fatso protein stained with ARG52285 anti-Fatso antibody [5-2H10].

Clone References

FTO levels affect RNA modification and the transcriptome.

Berulava T et al.
Eur J Hum Genet.,  (2013)