ARG52280

anti-Fibrillarin antibody [38F3]

anti-Fibrillarin antibody [38F3] for ICC/IF,IHC-Frozen sections,Western blot and C. elegans,D. melanogaster ,Human,Mouse,Plant,S. pombe

Gene Regulation antibody; Nucleolar Marker antibody; DFC Marker antibody; Dense fibrillar component Marker antibody
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Overview

Product Description

Mouse Monoclonal antibody [38F3] recognizes Fibrillarin

Tested Reactivity Hu, Ms, Ce, Dm, Plnt, S. pombe
Tested Application ICC/IF, IHC-Fr, WB
Host Mouse
Clonality Monoclonal
Clone 38F3
Isotype IgG1
Target Name Fibrillarin
Antigen Species Yeast
Immunogen Yeast nuclear preparations
Epitope EYRAWNPFRSKLAAAILGGV
Conjugation Un-conjugated
Alternate Names rRNA 2'-O-methyltransferase fibrillarin; RNU3IP1; 34 kDa nucleolar scleroderma antigen; FIB; FLRN; EC 2.1.1.-; Histone-glutamine methyltransferase

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:100
IHC-Fr1:100
WB1:500 - 1:1000
Application Note Specific for the ~34kDa Fibrillarin /Nop1p protein.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Total IgG fraction
Buffer Total IgG fraction and 10 mM Sodium azide
Preservative 10 mM Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 14113 Mouse FBL

GeneID: 2091 Human FBL

Swiss-port # P22087 Human rRNA 2'-O-methyltransferase fibrillarin

Swiss-port # P35550 Mouse rRNA 2'-O-methyltransferase fibrillarin

Gene Symbol NOP1
Background Nop1p was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae. The Nop1p protein is 327 amino acids in size (34.5kDa), is essential for yeast viability, and is localized in the nucleoli . The systematic name for S. cerevisiae Nop1 is YDL014W, and it is now known to be part of the small subunit processome complex, involved in the processing of pre-18S ribosomal RNA. Nop1p is the yeast homologue of a protein found in all eukaryotes and archea generally called fibrillarin . Fibrillarin/Nop1p is extraordinarily conserved, so that the yeast and human proteins are 67% identical, and the human protein can functionally replace the yeast protein. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a ~34kDa protein which was subsequently found to be fibrillarin. Recent studies show that knock-out of the fibrillarin gene in mice results in embryonic lethality, although mice with only one functional fibrillarin/Nop1p gene were viable . This antibody is becoming widely used as a convenient marker for nucleoli in a wide variety of species (e.g. 4-6).
Highlight Related Antibody Duos and Panels:
ARG30303 Nucleolar Marker Antibody Duo
Related products:
Fibrillarin antibodies; Fibrillarin Duos / Panels; Anti-Mouse IgG secondary antibodies;
Related poster download:
Organelle Markers & Loading Control
Research Area Gene Regulation antibody; Nucleolar Marker antibody; DFC Marker antibody; Dense fibrillar component Marker antibody
Calculated MW 34 kDa
PTM By homology to other fibrillarins, some or all of the N-terminal domain arginines are modified to asymmetric dimethylarginine (DMA).

Images (4) Click the Picture to Zoom In

  • ARG52280 anti-Fibrillarin antibody [38F3] ICC/IF image

    Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG52280 anti-Fibrillarin antibody [38F3] at 1:500 dilution. Left: primary antibody (green). Middle: DAPI (blue). Right: Merge.

    Secondary antibody: ARG55393 Goat anti-Mouse IgG (H+L) antibody (FITC)

  • ARG52280 anti-Fibrillarin antibody [38F3] WB image

    Western blot: 30 µg of HeLa cell lysate stained with ARG52280 anti-Fibrillarin antibody [38F3] at 1:1000 dilution.

  • ARG52280 anti-Fibrillarin antibody [38F3] ICC/IF image

    Immunofluorescence: HeLa cells stained with ARG52280 anti-Fibrillarin antibody [38F3] (green) at 1:100 dilution, and costained with ARG52468 anti-Vimentin antibody (red) at 1:1000 dilution. DAPI (blue) for nuclear staining.

    The Fibrillarin antibody shows strong staining of nucleoli in the nucleus, while the Vimentin antibody reveals cytoplasmic intermediate filaments.

  • ARG52280 anti-Fibrillarin antibody [38F3] WB image

    Western blot: HEK293 cytosol, HEK293 nuclear, NIH/3T3 cytosol and NIH/3T3 nuclear fractions stained with ARG52280 anti-Fibrillarin antibody [38F3] (green) at 1:500 dilution.

Customer's Feedback

nuts_pic      Excellent

Review for anti-Fibrillarin antibody [38F3]

Application:IF/ICC

Sample:HeLa

Fixation Buffer:100% Methanol

Fixation Time:10 min

Fixation Temperature:RT ºC

Permeabilization Buffer:0.1% Triton X-100

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Conjugation of Secondary Antibody:FITC

nuts_pic      Excellent

Review for anti-Fibrillarin antibody [38F3]

Application:IF/ICC

Sample:HeLa

Fixation Buffer:100% Methanol

Fixation Time:10 min

Fixation Temperature:RT ºC

Permeabilization Buffer:0.1% Triton X-100

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Conjugation of Secondary Antibody:FITC

nuts_pic      Excellent

Review for anti-Fibrillarin antibody [38F3]

Application:WB

Sample:HeLa

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Clone References

A novel mechanism inducing genome instability in Kaposi's sarcoma-associated herpesvirus infected cells.

WB / 

Jackson BR et al.
PLoS Pathog.,  (2014)

publication_link

 

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The transcription factor EGR1 localizes to the nucleolus and is linked to suppression of ribosomal precursor synthesis.

ICC/IF / Human

Ponti D et al.
PLoS One.,  (2014)

publication_link

 

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Def functions as a cell autonomous factor in organogenesis of digestive organs in zebrafish.

IHC-Fr / 

Tao T et al.
PLoS One.,  (2013)

publication_link

 

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Involvement of phosphatidylinositol 4,5-bisphosphate in RNA polymerase I transcription.

ICC/IF / Human

Yildirim S et al.
J Cell Sci.,  (2013)

publication_link

 

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Arabidopsis DEAD-box RNA helicase UAP56 interacts with both RNA and DNA as well as with mRNA export factors.

ICC/IF / Arabidopsis thaliana

Kammel C et al.
PLoS One.,  (2013)

publication_link

 

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Developmental regulation of nucleolus size during Drosophila eye differentiation.

IHC / Fruit fly (Drosophila melanogaster)

Baker NE et al.
PLoS One.,  (2013)

publication_link

 

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40S ribosome biogenesis co-factors are essential for gametophyte and embryo development.

IHC / Plant

Missbach S et al.
PLoS One.,  (2013)

publication_link

 

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MicroRNA miR-308 regulates dMyc through a negative feedback loop in Drosophila.

WB / Fruit fly (Drosophila melanogaster)

Daneshvar K et al.
Biol Open.,  (2013)

publication_link

 

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