ARG21971

anti-Fibrillin 1 antibody [11C1.3] (Biotin)

anti-Fibrillin 1 antibody [11C1.3] (Biotin) for IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,ICC/IF,Electron microscopy,Western blot and Human,Bovine

Overview

Product Description Biotin-conjugated Mouse Monoclonal antibody [11C1.3] recognizes Fibrillin 1
Tested Reactivity Hu, Bov
Tested Application EM, ICC/IF, IHC-Fr, IHC-P, WB
Specificity Human/Bovine/Japanese Macaque fibrillin-1
Host Mouse
Clonality Monoclonal
Clone 11C1.3
Isotype IgG1, kappa
Target Name Fibrillin 1
Antigen Species Bovine
Immunogen Microfibrils from the zonular apparatus of Bovine eye
Conjugation Biotin
Alternate Names ECTOL1; MFS1; WMS; SGS; SSKS; MASS; GPHYSD2; WMS2; ACMICD; OCTD; Fibrillin-1; FBN

Application Instructions

Application Suggestion
Tested Application Dilution
EMAssay-dependent
ICC/IFAssay-dependent
IHC-Fr< 4 µg/ml
IHC-P< 4 µg/ml
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer PBS and 0.1% Sodium azide.
Preservative 0.1% Sodium azide
Concentration 0.2 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2200 Human FBN1

GeneID: 281154 Bovine FBN1

Swiss-port # P35555 Human Fibrillin-1

Swiss-port # P98133 Bovine Fibrillin-1

Gene Symbol FBN1
Gene Full Name fibrillin 1
Background This gene encodes a member of the fibrillin family. The encoded protein is a large, extracellular matrix glycoprotein that serve as a structural component of 10-12 nm calcium-binding microfibrils. These microfibrils provide force bearing structural support in elastic and nonelastic connective tissue throughout the body. Mutations in this gene are associated with Marfan syndrome, isolated ectopia lentis, autosomal dominant Weill-Marchesani syndrome, MASS syndrome, and Shprintzen-Goldberg craniosynostosis syndrome. [provided by RefSeq, Jul 2008]
Function Fibrillins are structural components of 10-12 nm extracellular calcium-binding microfibrils, which occur either in association with elastin or in elastin-free bundles. Fibrillin-1-containing microfibrils provide long-term force bearing structural support. Regulates osteoblast maturation by controlling TGF-beta bioavailability and calibrating TGF-beta and BMP levels, respectively. [UniProt]
Calculated MW 312 kDa
PTM Fibrillin-1: Cleavage of N- and C-terminus by furin is required for incorporation into the extracellular matrix and assembly into microfibrils (PubMed:27026396). The C-terminus, which corresponds to the Asprosin chain, was initially thought to constitute a propeptide (PubMed:24982166). Fibrillin-1 and Asprosin chains are still linked together during the secretion from cells, but are subsequently separated by furin, an essential step for incorporation of Fibrillin-1 into the nascent microfibrils (PubMed:24982166).
Fibrillin-1: Forms intermolecular disulfide bonds either with other fibrillin-1 molecules or with other components of the microfibrils.