ARG23998

anti-Fibronectin antibody

anti-Fibronectin antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,ELISA and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes Fibronectin
Tested Reactivity Hu
Tested Application ELISA, ICC/IF, IHC-Fr, IHC-P
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Fibronectin
Antigen Species Human
Immunogen Fibronectin extracted from Human plasma.
Conjugation Un-conjugated
Alternate Names ED-B; CIG; GFND; Cold-insoluble globulin; FNZ; LETS; GFND2; Fibronectin; MSF; FINC; FN

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA1:2000
ICC/IF1:40
IHC-Fr1:500
IHC-P1:500
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified.
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2335 Human FN1

Swiss-port # P02751 Human Fibronectin

Gene Symbol FN1
Gene Full Name fibronectin 1
Background This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. The encoded preproprotein is proteolytically processed to generate the mature protein. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. The full-length nature of some variants has not been determined. [provided by RefSeq, Jan 2016]
Function Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.

Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. [UniProt]
Cellular Localization Secreted, extracellular space, extracellular matrix. [UniProt]
Highlight Related products:
Fibronectin antibodies; Fibronectin ELISA Kits; Fibronectin Duos / Panels; Anti-Rabbit IgG secondary antibodies;
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Calculated MW 272 kDa
PTM Sulfated.

It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.

Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).

Phosphorylated by FAM20C in the extracellular medium.

Proteolytic processing produces the C-terminal NC1 peptide, anastellin.

Some lysine residues are oxidized to allysine by LOXL3, promoting fibronectin activation and matrix formation. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG23998 anti-Fibronectin antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human skin tissue stained with ARG23998 anti-Fibronectin antibody.