ARG42045

anti-Fibronectin antibody

anti-Fibronectin antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,ICC/IF and Human,Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes Fibronectin
Tested Reactivity Hu, Ms
Predict Reactivity Rat
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Fibronectin
Antigen Species Human
Conjugation Un-conjugated
Alternate Names ED-B; CIG; GFND; Cold-insoluble globulin; FNZ; LETS; GFND2; Fibronectin; MSF; FINC; FN

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:200 - 1:500
IHC-P1:100 - 1:200
WB1:500
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse plasma

Properties

Form Liquid
Purification Affinity purified.
Buffer 100 mM Tris Glycine (pH 7.0), 0.025% ProClin 300 and 20% Glycerol.
Preservative 0.025% ProClin 300
Stabilizer 20% Glycerol
Concentration 1.45 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 14268 Mouse FN1

GeneID: 2335 Human FN1

Swiss-port # P02751 Human Fibronectin

Swiss-port # P11276 Mouse Fibronectin

Gene Symbol FN1
Gene Full Name fibronectin 1
Background This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]
Function Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.

Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. [UniProt]
Cellular Localization Secreted, extracellular space, extracellular matrix. [UniProt]
Highlight Related products:
Fibronectin antibodies; Fibronectin ELISA Kits; Fibronectin Duos / Panels; Anti-Rabbit IgG secondary antibodies;
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Calculated MW 272 kDa
PTM Sulfated.

It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.

Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).

Phosphorylated by FAM20C in the extracellular medium.

Proteolytic processing produces the C-terminal NC1 peptide, anastellin.

Some lysine residues are oxidized to allysine by LOXL3, promoting fibronectin activation and matrix formation. [UniProt]

Images (3) Click the Picture to Zoom In

  • ARG42045 anti-Fibronectin antibody ICC/IF image

    Immunofluorescence: Cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT, then blocked with 5% BSA for 30 min at RT. Cells were stained with ARG42045 anti-Fibronectin antibody (red) at 1:200 dilution and 4°C. DAPI (blue) for nuclear staining.

  • ARG42045 anti-Fibronectin antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human breast cancer tissue stained with ARG42045 anti-Fibronectin antibody at 1:100 dilution.

  • ARG42045 anti-Fibronectin antibody WB image

    Western blot: 60 µg of Mouse plasma lysate stained with ARG42045 anti-Fibronectin antibody at 1:500 dilution, overnight at 4°C.