ARG56127

anti-Fibronectin antibody [FN12-8]

anti-Fibronectin antibody [FN12-8] for IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Inhibition assay,Western blot and Bovine,Human

Overview

Product Description

Mouse Monoclonal antibody [FN12-8] recognizes Fibronectin

Tested Reactivity Hu, Bov
Tested Application IHC-Fr, IHC-P, Inhib, WB
Specificity This antibody recognizes an epitope in the cell-binding domain of Human and Bovine fibronectin and can inhibit cell adhesion.
Host Mouse
Clonality Monoclonal
Clone FN12-8
Isotype IgG1
Target Name Fibronectin
Antigen Species Human
Immunogen Human plasma fibronectin
Conjugation Un-conjugated
Full Name fibronectin 1
Alternate Names ED-B; CIG; GFND; Cold-insoluble globulin; FNZ; LETS; GFND2; Fibronectin; MSF; FINC; FN

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-Fr1 - 10 µg/ml
IHC-P1 - 10 µg/ml
Inhib0.1 - 1.0 µg/ml on 10 µg/ml fibronectin-coated microwells
WB2 - 10 µg/ml
Application Note IHC-P: Antigen Retrieval: By Proteinase K.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 10 mM PBS (pH 7.4) and 1% BSA
Stabilizer 1% BSA
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 2335 Human FN1

Swiss-port # P02751 Human Fibronectin

Gene Symbol FN1
Gene Full Name fibronectin 1
Background This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]
Function Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.

Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. [UniProt]
PTM Sulfated.
It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
Phosphorylated by FAM20C in the extracellular medium.
Proteolytic processing produces the C-terminal NC1 peptide, anastellin.
Some lysine residues are oxidized to allysine by LOXL3, promoting fibronectin activation and matrix formation.

Images (1) Click the Picture to Zoom In

  • ARG56127 anti-Fibronectin antibody [FN12-8] IHC-P image

    Immunohistochemistry: Human well-differentiated colon cancer tissue (left) and moderately-differentiated colon cancer (right). Tissues were stained with ARG56127 anti-Fibronectin antibody [FN12-8].