anti-GABAA Receptor alpha 2 antibody
anti-GABAA Receptor alpha 2 antibody for Western blot and Mouse,Rat
|Product Description||Rabbit Polyclonal antibody recognizes GABAA Receptor alpha 2|
|Tested Reactivity||Ms, Rat|
|Target Name||GABAA Receptor alpha 2|
|Immunogen||Fusion protein from the cytoplasmic loop of the alpha 2 subunit|
|Alternate Names||A; Gamma-aminobutyric acid receptor subunit alpha-2; GABA|
|Application Note||Specific for the ~51k α2-subunit of the GABAA receptor in Western blots. Labeling is absent in 2-subunit knockout animals.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|Buffer||10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol|
|Stabilizer||0.1 mg/ml BSA, 50% Glycerol|
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Background||Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl− channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six s, four s and four s, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for - and -subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a -subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different - subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003).|
|Research Area||Neuroscience antibody|
|Calculated MW||51 kDa|
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ARG52288 anti-GABAA Receptor alpha 2 antibody WB image
Western blot: mouse brain lysates from wild type (Control) and α2-knockout (α2-K/O) animals stained with ARG52288 anti-GABAA Receptor alpha 2 antibody showing specific immunolabeling of the ~51k α2-subunit of the GABAA-R. The labeling was absent from a lysate prepared from α2-knockout animals.