ARG52289

anti-GABAA Receptor alpha 3 antibody

anti-GABAA Receptor alpha 3 antibody for Western blot,Immunohistochemistry and Mouse,Rat

Neuroscience antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes GABAA Receptor alpha 3
Tested Reactivity Ms, Rat
Predict Reactivity Hu, Bov, Dog, NHuPrm, Zfsh
Tested Application IHC, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name GABAA Receptor alpha 3
Antigen Species Rat
Immunogen Synthetic peptide from the N-terminal region of the alpha 3 subunit
Conjugation Un-conjugated
Alternate Names A; Gamma-aminobutyric acid receptor subunit alpha-3; GABA

Application Instructions

Application Suggestion
Tested Application Dilution
IHC1:1,00
WB1:1,000
Application Note Specific for the ~51k α3-subunit of the GABAA receptor in Western blots. Labeling is absent in α3-subunit knockout animals.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity Purified
Buffer 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol
Stabilizer 0.1 mg/ml BSA, 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 14396 Mouse GABRA3

GeneID: 24947 Rat GABRA3

Swiss-port # P26049 Mouse Gamma-aminobutyric acid receptor subunit alpha-3

Gene Symbol GABRA3
Gene Full Name gamma-aminobutyric acid (GABA) A receptor, alpha 3
Background Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Clchannel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six αs, four βs and four γs, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for α- and β-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a γ-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different α- subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003).
Research Area Neuroscience antibody
Calculated MW 55 kDa

Images (2) Click the Picture to Zoom In

  • ARG52289 anti-GABAA Receptor alpha 3 antibody WB image

    Western Blot: Rat brain lysates from wild type (Control) and a3-knockout (a3-K/O) animals showing specific immunolabeling of the ~51k a3-subunit of the GABAA-R stained with GABA A Receptor alpha 3 Antibody (ARG52289). The labeling was absent from a lysate prepared from a3-knockout animals.

  • ARG52289 anti-GABAA Receptor alpha 3 antibody IHC image

    Immunohistochemistry: rat amygdala stained with ARG52289 anti-GABAA Receptor alpha 3 antibody showing labeling of GABAA alpha 3 subunit.