anti-GABAA Receptor beta 3 phospho (Ser408 / Ser409) antibody
anti-GABAA Receptor beta 3 phospho (Ser408 / Ser409) antibody for Western blot and Rat
|Product Description||Rabbit Polyclonal antibody recognizes GABAA Receptor beta 3 phospho (Ser408 / Ser409)|
|Predict Reactivity||Hu, Ms, Bov, Chk, Dog, NHuPrm, Xenopus laevis, Zfsh|
|Target Name||GABAA Receptor beta 3|
|Immunogen||Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser408/409 conjugated to KLH|
|Alternate Names||Gamma-aminobutyric acid receptor subunit beta-3; A; ECA5; GABA|
|Application Note||Specific for the ~53k GABAA receptor β3 subunit phosphorylated at Ser408/409. Immunolabeling of the GABAA band is completely blocked by the phospho-peptide used as antigen but not by the corresponding dephospho-peptide.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|Buffer||10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol|
|Stabilizer||0.1 mg/ml BSA, 50% Glycerol|
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||gamma-aminobutyric acid (GABA) A receptor, beta 3|
|Background||Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system. There are two major classes of GABA receptors: the GABAA and the GABAB subtype of receptors. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six αs, four βs and four γs, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for α- and β-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a γ-subunit is required for benzodiazepine modulation. Phosphorylation of serine 408 and serine 409 within the β3 subunit have been shown to be critical for the functional modulation of β3 containing recombinant receptors (Brandon et al., 2000).|
|Research Area||Neuroscience antibody|
|Calculated MW||54 kDa|
Images (1) Click the Picture to Zoom In
ARG52298 anti-GABAA Receptor beta 3 phospho (Ser408 / Ser409) antibody WB image
Western blot: Rat hippocampal lysate showing specific immunolabeling of the ~53k GABAA beta 3 protein phosphorylated at Ser 408/409 (control) stained with ARG52298 anti-GABAA Receptor beta 3 phospho (Ser408 / Ser409) antibody. Immunolabeling is blocked by the phospho-peptide (peptide) used as antigen.