anti-GABAA Receptor gamma 2 antibody
anti-GABAA Receptor gamma 2 antibody for Western blot,Immunohistochemistry and Rat
|Product Description||Rabbit Polyclonal antibody recognizes GABAA Receptor gamma 2|
|Predict Reactivity||Hu, Ms, Chk, NHuPrm, Zfsh|
|Tested Application||IHC, WB|
|Target Name||GABAA Receptor gamma 2|
|Immunogen||Synthetic peptide corresponding to amino acid residues specific to the gamma 2 subunit conjugated to KLH|
|Alternate Names||A; CAE2; ECA2; GEFSP3; Gamma-aminobutyric acid receptor subunit gamma-2; GABA|
|Application Note||Specific for the ~46k γ2-subunit of the GABAA receptor in Western blots of Rat brain extracts.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||gamma-aminobutyric acid (GABA) A receptor, gamma 2|
|Background||Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl− channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six αs, four βs and four γs, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for α- and β-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, co-expression of a γ-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different α- subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003). Lastly, phosphorylation of β-subunits of the receptor has been shown to modulate GABAA-R function (Brandon et al., 2003).|
|Research Area||Neuroscience antibody|
|Calculated MW||54 kDa|
|PTM||Palmitoylated by ZDHHC3/GODZ; which may affect presynaptic clustering and/or cell surface stability.|
Images (2) Click the Picture to Zoom In
ARG52301 anti-GABAA Receptor gamma 2 antibody WB image
Western blot: 10 μg of rat hippocampal lysate stained with ARG52301 anti-GABAA Receptor gamma 2 antibody showing immunolabeling of the ~46k γ2-subunit of the GABAA-R.
ARG52301 anti-GABAA Receptor gamma 2 antibody IHC image
Immunohistochemistry: rat cerebellum stained with ARG52301 anti-GABAA Receptor gamma 2 antibody showing labeling of GABAA g2 subunit in fuchsia.