ARG52301

anti-GABAA Receptor gamma 2 antibody

anti-GABAA Receptor gamma 2 antibody for Western blot,Immunohistochemistry and Rat

Neuroscience antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes GABAA Receptor gamma 2
Tested Reactivity Rat
Predict Reactivity Hu, Ms, Chk, NHuPrm, Zfsh
Tested Application IHC, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name GABAA Receptor gamma 2
Antigen Species Rat
Immunogen Synthetic peptide corresponding to amino acid residues specific to the gamma 2 subunit conjugated to KLH
Conjugation Un-conjugated
Alternate Names A; CAE2; ECA2; GEFSP3; Gamma-aminobutyric acid receptor subunit gamma-2; GABA

Application Instructions

Application Suggestion
Tested Application Dilution
IHC1:400
WB1:1,000
Application Note Specific for the ~46k γ2-subunit of the GABAA receptor in Western blots of Rat brain extracts.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Neat Serum
Buffer Neat serum
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 29709 Rat GABRG2

Swiss-port # P18508 Rat Gamma-aminobutyric acid receptor subunit gamma-2

Gene Symbol GABRG2
Gene Full Name gamma-aminobutyric acid (GABA) A receptor, gamma 2
Background Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl− channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six αs, four βs and four γs, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for α- and β-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, co-expression of a γ-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different α- subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003). Lastly, phosphorylation of β-subunits of the receptor has been shown to modulate GABAA-R function (Brandon et al., 2003).
Research Area Neuroscience antibody
Calculated MW 54 kDa
PTM Palmitoylated by ZDHHC3/GODZ; which may affect presynaptic clustering and/or cell surface stability.

Images (2) Click the Picture to Zoom In

  • ARG52301 anti-GABAA Receptor gamma 2 antibody WB image

    Western blot: 10 μg of rat hippocampal lysate stained with ARG52301 anti-GABAA Receptor gamma 2 antibody showing immunolabeling of the ~46k γ2-subunit of the GABAA-R.

  • ARG52301 anti-GABAA Receptor gamma 2 antibody IHC image

    Immunohistochemistry: rat cerebellum stained with ARG52301 anti-GABAA Receptor gamma 2 antibody showing labeling of GABAA g2 subunit in fuchsia.