ARG22242

anti-GABAB Receptor 2 antibody [S81-2]

anti-GABAB Receptor 2 antibody [S81-2] for Western blot,Immunohistochemistry,ICC/IF and Human,Mouse,Rat

Overview

Product Description Mouse Monoclonal antibody [S81-2] recognizes GABAB Receptor 2
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC, WB
Specificity Detects ~105kDa. No cross-reactivity against GABA(B)R1.
Host Mouse
Clonality Monoclonal
Clone S81-2
Isotype IgG1
Target Name GABAB Receptor 2
Antigen Species Rat
Immunogen Fusion protein around aa. 861-912 of Rat GABA B Receptor 2
Conjugation Un-conjugated
Alternate Names HRIHFB2099; GABA-B receptor 2; GABA-B-R2; GPRC3B; GABA-BR2; G-protein coupled receptor 51; GPR51; GABABR2; Gamma-aminobutyric acid type B receptor subunit 2; HG20; Gb2

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IFAssay-dependent
IHCAssay-dependent
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol
Preservative 0.09% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 242425 Mouse GABBR2

GeneID: 83633 Rat GABBR2

GeneID: 9568 Human GABBR2

Gene Symbol Gabbr2
Gene Full Name gamma-aminobutyric acid (GABA) B receptor 2
Background The multi-pass membrane protein encoded by this gene belongs to the G-protein coupled receptor 3 family and GABA-B receptor subfamily. The GABA-B receptors inhibit neuronal activity through G protein-coupled second-messenger systems, which regulate the release of neurotransmitters, and the activity of ion channels and adenylyl cyclase. This receptor subunit forms an active heterodimeric complex with GABA-B receptor subunit 1, neither of which is effective on its own. Allelic variants of this gene have been associated with nicotine dependence.[provided by RefSeq, Jan 2010]
Function Component of a heterodimeric G-protein coupled receptor for GABA, formed by GABBR1 and GABBR2. Within the heterodimeric GABA receptor, only GABBR1 seems to bind agonists, while GABBR2 mediates coupling to G proteins. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipid hydrolysis. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA receptor inhibits neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA receptor decreases neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception. [UniProt]
Cellular Localization Cell Junction, Cell membrane, postsynaptic cell membrane, Synapse
Calculated MW 106 kDa

Images (2) Click the Picture to Zoom In

  • ARG22242 anti-GABAB Receptor 2 antibody [S81-2] ICC/IF image

    Immunofluorescence: Human Neuroblastoma cell line SK-N-BE. Fixation: 4% Formaldehyde for 15 min at RT. Primary antibody: ARG22242 anti-GABAB Receptor 2 antibody [S81-2] at 1:100 for 60 min at RT. Secondary antibody: Goat anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Primary antibody (D) Composite.

  • ARG22242 anti-GABAB Receptor 2 antibody [S81-2] WB image

    Western blot: 10 µg of Rat Brain Membrane. Block: 5% milk. Primary antibody: ARG22242 anti-GABAB Receptor 2 antibody [S81-2] at 1:1000 for 1 hour at RT. Secondary antibody: Goat anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT.