anti-GAP43 antibody [1E3]
anti-GAP43 antibody [1E3] for Western blot,ICC/IF,IHC-Frozen sections and Human,Horse,Cow,Pig,Rat,Mouse
|Product Description||Mouse Monoclonal antibody [1E3] recognizes GAP43|
|Tested Reactivity||Hu, Ms, Rat, Cow, Hrs, Pig|
|Tested Application||ICC/IF, IHC-Fr, WB|
|Immunogen||C-terminal peptide of Mouse and Rat GAP43, which is KEDPEADQEHA, with an N-terminal Cys added to allow chemical coupling to KLH carrier protein.|
|Alternate Names||pp46; Growth-associated protein 43; B-50; Neuromodulin; PP46; Axonal membrane protein GAP-43; Neural phosphoprotein B-50|
|Application Note||* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.|
|Buffer||PBS and 50% Glycerol.|
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||growth associated protein 43|
|Background||The protein encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This protein is considered a crucial component of an effective regenerative response in the nervous system. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]|
|Function||This protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction. [UniProt]|
|Calculated MW||25 kDa|
|PTM||Phosphorylated at Ser-41 by PHK. Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity.
Palmitoylation by ARF6 is essential for plasma membrane association and axonal and dendritic filopodia induction. Deacylated by LYPLA2.
Images (2) Click the Picture to Zoom In
ARG10714 anti-GAP43 antibody [1E3] ICC/IF image
Immunocytochemistry: Mixed neuronal cultures stained with ARG10714 anti-GAP43 antibody [1E3] (green) and co-stained with rabbit antibody to MAP2 (red); DNA (blue). The GAP43 antibody stains the plasma membrane of neurons and is particularly concentrated in dendrites.
ARG10714 anti-GAP43 antibody [1E3] WB image
Western blot: Whole Rat spinal cord lysates stained with ARG10714 anti-GAP43 antibody [1E3]. Dots in middle of strips indicate position of 50 kDa and 37 kDa protein bands.