ARG10716

anti-GAP43 antibody [5E8]

anti-GAP43 antibody [5E8] for ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse,Rat

Overview

Product Description Mouse Monoclonal antibody [5E8] recognizes GAP43
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-Fr, WB
Host Mouse
Clonality Monoclonal
Clone 5E8
Isotype IgG1
Target Name GAP43
Antigen Species Human
Immunogen Recombinant full-length Human GAP43.
Conjugation Un-conjugated
Full Name growth associated protein 43
Alternate Names pp46; Growth-associated protein 43; B-50; Neuromodulin; PP46; Axonal membrane protein GAP-43; Neural phosphoprotein B-50

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:1000 - 1:5000
IHC-Fr1:1000 - 1:5000
WB1:1000 - 1:5000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification.
Buffer PBS and 50% Glycerol.
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 14432 Mouse GAP43

GeneID: 2596 Human GAP43

GeneID: 29423 Rat GAP43

Gene Symbol GAP43
Gene Full Name growth associated protein 43
Background The protein encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This protein is considered a crucial component of an effective regenerative response in the nervous system. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Function This protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction. [UniProt]
PTM Phosphorylated at Ser-41 by PHK. Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity.
Palmitoylation by ARF6 is essential for plasma membrane association and axonal and dendritic filopodia induction. Deacylated by LYPLA2.

Images (4) Click the Picture to Zoom In

  • ARG10716 anti-GAP43 antibody [5E8] ICC/IF image

    Immunocytochemistry: Mixed Rat neuronal cultures stained with ARG10716 anti-GAP43 antibody [5E8] (green). The GAP43 antibody stains the plasma membrane of neurons and is particularly concentrated in dendrites.

  • ARG10716 anti-GAP43 antibody [5E8] ICC/IF image

    Immunofluorescence: Cortical neuron-glial cells from E20 Rat stained with ARG10716 anti-GAP43 antibody [5E8] (red) at 1:1000 dilution and costained with ARG52313 anti-GFAP antibody (green) at 1:2000 dilution. DAPI (blue) for nuclear staining.

    The GAP43 antibody labels protein expressed in the axonal membrane of neuronal cells, while the GFAP antibody stains astroglial cells.

  • ARG10716 anti-GAP43 antibody [5E8] WB image

    Western blot: 1) SH-SY5Y cell lysate, and  2) whole Rat brain lysate was stained with ARG10716 anti-GAP43 antibody [5E8] at 1: 2000 dilution.

  • ARG10716 anti-GAP43 antibody [5E8] WB image

    Western blot: Rat brain, Rat spinal cord, Mouse brain, Mouse spinal cord, C6 and SH-SY5Y cell lysates stained with ARG10716 anti-GAP43 antibody [5E8] (green) at 1:2000 dilution.

    The protein is expressed in rodent and Human neurons and neuronal derived cells but not in C6 cells which are of glial origin.