ARG10735

anti-GAP43 antibody

anti-GAP43 antibody for ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse,Rat,Cow,Horse,Pig

Overview

Product Description Rabbit Polyclonal antibody recognizes GAP43
Tested Reactivity Hu, Ms, Rat, Cow, Hrs, Pig
Predict Reactivity Chk
Tested Application ICC/IF, IHC-Fr, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name GAP43
Species Mouse
Immunogen C-terminal peptide of Mouse and Rat GAP43, which is KEDPEADQEHA, with an N-terminal Cys added to allow chemical coupling to KLH carrier protein.
Conjugation Un-conjugated
Full Name growth associated protein 43
Alternate Names pp46; Growth-associated protein 43; B-50; Neuromodulin; PP46; Axonal membrane protein GAP-43; Neural phosphoprotein B-50

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:1000
IHC-Fr1:1000
WB1:10000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 25 kDa

Properties

Form Liquid
Purification Affinity purification.
Buffer PBS and 50% Glycerol.
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 14432 Mouse GAP43

GeneID: 2596 Human GAP43

GeneID: 29423 Rat GAP43

Gene Symbol Gap43
Gene Full Name growth associated protein 43
Background The protein encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This protein is considered a crucial component of an effective regenerative response in the nervous system. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Function This protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction. [UniProt]
PTM Phosphorylated at Ser-41 by PHK. Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity.
Palmitoylation by ARF6 is essential for plasma membrane association and axonal and dendritic filopodia induction. Deacylated by LYPLA2.

Images (4) Click the Picture to Zoom In

  • ARG10735 anti-GAP43 antibody ICC/IF image

    Immunocytochemistry: Rat mixed neuron-glial cultures stained with ARG10735 anti-GAP43 antibody (red). Blue is DNA staining.

  • ARG10735 anti-GAP43 antibody ICC/IF image

    Immunofluorescence: Cortical neuron-glial cells from E20 Rat stained with ARG10735 anti-GAP43 antibody (green) at 1:2000 dilution and costained with Mouse mAb to vimentin (red) at 1:2000 dilution. DAPI (blue) for nuclear staining.

    The GAP43 antibody labels protein expressed in the axonal membrane of neuronal cells, while vimentin antibody stains intermediate filaments in fibroblasts and other non-neuronal cells.

  • ARG10735 anti-GAP43 antibody WB image

    Western blot: Homogenate of cow cerebellum stained with ARG10735 anti-GAP43 antibody.

  • ARG10735 anti-GAP43 antibody WB image

    Western blot: Rat brain, Rat spinal cord, Mouse brain, Mouse spinal cord, SH-SY5Y and C6 cell lysates stained with ARG10735 anti-GAP43 antibody (green) at 1:20000 dilution.

    The GAP43 protein is detected only in the lysates of neuronal origin. C6 cells are a Rat glioma cell line and do not express GAP43 protein.